Abstract: To synergistically increase crop yield and fertilizer nitrogen (N) efficiency, a field experiment was conducted to study effects of N application on dry matter accumulation and nutrients accumulation at different growing stages with Liaoxing 1 as the tested objective. The results showed that N application significantly increased rice dry matter and N, phosphorous (P) and potassium (K) accumulation and their accumulation rate, especially at the late growing stage. With rice growing, dry matter and N, P and K accumulation all increased, the accumulation rate was obviously high between jointing stage and milk stage for dry matter and N, while the increasing rate of P and K rose before milk stage, indicating that early growing stage was the key period for P and K uptake by rice, and late growing stage was the key period for N uptake and dry matter accumulation. In view of grain yield, fertilizer N efficiency and nutrients accumulation characteristics, N postponed application at the rate of 210 kg/hm² (40%base fertilizer + 40% tillering fertilize r+ 20% heading fertilizer) is the optimal N application strategy.