Abstract: In the present research, nine high lysine maize and two common hybrids were used as experiment materials, and quantitative determination of lysine contents was carried out by Hitachi L-8900Amino-acid Analyzer, to understand the effect of the analyzer in distinguishing high lysine maize variety and common maize variety. The results showed that the lysine contents of high lysine maize variety were 0.34%-0.42%, while of common maize variety were 0.24%-0.25%, and the difference was significant. Compared with other traditional methods, this method is rapid, simple, sensitive and needs less samples, and applicable in maize breeding.