Abstract: The study aims to establish an experimental system of rapid identification of beet variety purity and authenticity, to optimized extraction, detection of DNA, PCR reaction system, program, electrophoresis and the method of developing multiple aspects, and finally to set up a set of simple, rapid, economical and less polluted identification system for sugarbeet variety purity and authenticity. With 96- hole PCR plates, alkali decomposition method was used to extract DNA from dried seed (or leaf powder). Using nontoxic Gelred substitute carcinogenic EB, DNA concentration was extracted with λDNA. PCR reaction system was 5 μL, using multiple PCR to replace a single PCR; PCR reaction procedure was 94℃, degeneration 15 s, annealing 15 s, extension 72℃ for 30 s, 30 cycles and extended at 72℃ for 5 min finally. Separation of the PCR products was conducted using 8% of Native-PAGE, and rapid argentation was used to develop polyacrylamide gel after electrophoresis. Using the optimized program, a skillful laboratory operator can complete 192 samples of detection within one day.