Detection ofβ-Lactamase and 16S rRNA Methylase Gene in Resistant Escherichia coli Isolates from Cattle and Sheep in Xinjiang

doi:10.11924/j.issn.1000-6850.2014-0911

Abstract

Abstract: The research aims at the resistant Escherichia coli carryingβ- lactamases and or 16S rRNA methylase gene and its coexistence conditions in cattle and sheep farms in Xinjiang.β-lactamase and 16S rRNA methylase genes by PCR inβ-lactam-resitance Escherichia coli were isolated from sheep farm (168 isolates) and cattle farm (32 isolates). The products of PCR were analyzed by sequencing. The results showed that the blaTEM (42.26%, 71/168) and blaCTX-M (2.98%, 5/168) genes were mainlyβ-lactamase genes from sheep; blaTEM（15.63%，5/32）genes were mainlyβ-lactamase genes from cattle. blaCTX- M was not detected, and other resistance genotypes were not seized in the two farms. ArmA genes were not detected. The detected 16S rRNA methylase genes were rmtB gene from sheep and cattle isolates, its detection rate was 4.17% (7/168) and 50.00% (16/32). Coexistence gene: three (1.79%, 3/168) isolates from cattle were found to possess the blaTEM and blaCTX-M genes in the sheep farm. seven (4.17%, 7/168) isolates from sheep were found to possess the blaTEM and rmtB genes, and two (6.25%, 2/32) isolates were found to possess the blaTEM and rmtB genes in the cattle farm. The other detectedβ- lactamase and 16S rRNA methylase genes were not found in these farms. Escherichia coli isolates from cattle and sheep farms hadβ- lactamase and 16S rRNA methylase genes mediated bacterial resistance, and were dominated by blaTEM and rmtB genes in Xinjiang. In addition, different types of drug-resistant genes coexist in the same drug-resistance bacterial strain.

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