Abstract: Because of the exsiting Cucurbita moschata hybrid purity identification system based on SSR was complicated and difficult for the production of practice, a simple Cucurbita moschata hybrid purity identification system based on SSR was introduced here. Without grinding, freezing, centrifugation and drying, the whole genomic DNA extraction period is less than 3 minutes. Comparing to the conventional method, the PCR detection system and the silver stained steps saved time and cost, the whole process is less than 4 hours, one person a day can complete 480 hybrids detection easily, and the coincidence rate of the simple SSR-based purity with the field test results was 99%. This method would provide the base for large-scale rapid purity identification of Cucurbita moschata hybrids.