Abstract: The author studied effect on yield and quality of Houttuynia cordata by applying nitrogen and organic fertilizer in different dosage, which provided the basis for the cultivation measures to obtain high yields with fine qualities. Quadratic saturation D optimum design method was used to determine the yield and quality of Houttuynia cordata. Mathematical model was established based on the yield and quality of Houttuynia cordata by applying nitrogen and organic fertilizer in different dosage. Through model analysis and simulating optimization, when vine yield≥8000 kg/hm², rhizome yield≥31000 kg/hm², vine extract≥16.60% , protein contents of rhizome≥1.64% , soluble sugar of rhizome≥3.60% , fat contents of rhizome≥0.37% , cellulosic contents of rhizome≤3.28%, the optimization of cultivation measures was that the nitrogen application amount 182.51-490.47 kg/hm² and organic fertilizer application amount 20678.46-49802.34 kg/hm². The reasonable application of nitrogen fertilizer and organic fertilizer was beneficial for the improvement of its production and quality improvement in Houttuynia cordata cultivation.