Study on the Prevention and Cure of Browning and Contamination

doi:10.11924/j.issn.1000-6850.casb14110014

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (10): 33-38.doi: 10.11924/j.issn.1000-6850.casb14110014

Special Issue: 生物技术；园艺

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Study on the Prevention and Cure of Browning and Contamination

During Tissue Culture of Toxicodendron vernicifluum

Zhao Yueming¹, Zhao Yanming¹, He Chengzhong¹, Liao Shengxi², Li Dan³(¹Southwest Forestry University/Key Laboratory for Forest Resource Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Kunming 650224;²Research Institute of Resources Insects, CAF, Kunming 650224;³Yunnan Academy of Biodiversity/ Southwest Forestry University, Kunming 650224)

Received:2014-11-03 Revised:2015-03-05 Accepted:2014-12-24 Online:2015-05-05 Published:2015-05-05

Abstract

Abstract: As an important economic species, Toxicodendron vernicifluum could be propagated by the way of plant tissue culture. In order to solve the contamination, nystatin, amphotericin B, benzalkonium chloride, 75% ethanol and 0.1% mercuric chloride were used. Besides, cysteine, MS and White medium were used to solve browning. SPSS 12.0 statistical software was used for the single factor analysis of variance and Duncan's new multiple comparison analysis. By counting contamination rate, browning rate and survival rate, the best method of disinfection was 75% ethanol soaked 1 min, 1% benzalkonium soak 1 min, 0.1% mercuric chloride for 20 min, and the contamination rate was 1.15% . The best optimum medium was White culture mixed with 100 mg/L cysteine, and the browning rate was 1.15%. The survival rate was improved to 97.67%. The efficient and stable sterile system of Toxicodendron vernicifluum had been laid a solid foundation for the whole tissue culture system.

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