Initial Study on Lactic Acid Inhibiting the Apoptosis of IPEC-J2 Induced by TNF-alpha

doi:10.11924/j.issn.1000-6850.casb14120211

Abstract

Abstract: The purpose of this study was to determine whether lactic acid, the main product of lactobacillus, could inhibit the apoptosis of IPEC-J2 induced by TNF-alpha, and conduct an initial study on the specific mechanism of lactic acid in protecting the intestinal barrier. Four groups were created: TNF- alpha group, treated with TNF- alpha with the concentration of 200 μg/L; LAC group, treated with lactic acid with the concentration of 100 μL/L; LAC + TNF- alpha group, treated with TNF- alpha with the concentration of 200 μg/L and lactic acid with the concentration of 100 μL/L; control group, treated with maintenance medium. Cell vitality of each group was measured by MTT, proportion of caspase-3 and the vitality of caspase-3 in the total protein were detected by ELISA after 12 hours. The results showed that the cell vitality of TNF-alpha group was significantly lower than that of the control group (P＜0.01), while cell vitality of LAC+TNF-alpha group was significantly higher than that of TNF-alpha group (P＜0.05). The proportion of caspase-3 and its vitality in the total protein of the LAC+TNF-alpha group were lower than those of TNF-alpha group(P＜0.05). Thus it came to a conclusion that the lactic acid could inhibit the cellular damage induced by TNF-alpha as well as down- regulate the expression and vitality of caspase- 3. The results implied that lactic acid might protect the intestinal epithelium through inhibiting the apoptosis of the epithelial cells.

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