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Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (13): 1-4.doi: 10.11924/j.issn.1000-6850.casb14110155

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Polyploidy Induction of Pteroceltis tatarinowii Maxim.

张 林 and 王峰   

  • Received:2014-11-24 Revised:2015-01-13 Accepted:2015-01-25 Online:2015-06-02 Published:2015-06-02

Abstract: In order to obtain tetraploid Pteroceltis tatarinowii Maxim. with excellent ornamental traits, the young seedlings were used as materials, the stem apex growing point of Pteroceltis tatarinowii Maxim. was treated with different concentrations of colchicine solution at different hours to figure out the induction effects. The results showed that: the most effective induction was obtained by 0.6%-0.8% colchicine treatment for 72 h with 34.2% mutation rate. By flow cytometric appraisal and chromosome observation of the stem apex growing point of Pteroceltis tatarinowii Maxim., the tetraploid plants were successfully obtained with the chromosome number 2n=4x=36.