Abstract: In order to compare the difference of photosynthetic characteristics between female and male Actinidia arguta, the wild female and male Actinidia arguta of mature plant were used as materials and the photosynthetic characteristics were measured by the CI-340 portable photosynthetic system. The results stated that: the daily variation of net photosynthesis rate of female Actinidia arguta appeared a bimodal peak, on the contrary, the male Actinidia arguta showed a triple one. However, both female and male showed obvious characteristics of photosynthesis mid-nap; the daily variation of transpiration rate of female Actinidia arguta revealed a unimodal peak while a bimodal peak could be seen in the male Actinidia arguta. Nevertheless, both female and male showed less obvious characteristics of transpiration mid- nap; the diurnal variations of stomatal conductance of female and male Actinidia arguta demonstrated the same phenomenon as net photosynthesis rate; the curvilinear trend of diurnal variations of internal CO2 between female and male Actinidia arguta was basically consistent. And both of them showed three peaks whose time and values were different; the diurnal variations of leaf temperature of female and male Actinidia arguta both showed a bimodal peak, but the time and value of it had significant differences. The photosynthetic characteristics of female and male Actinidia arguta have a significant difference which includes not only the daily variation tendency, but also the value of the peak, so it can be used as an effective method for sex identification of Actinidia arguta.