Abstract: In order to grasp the occurrence and harm rule of root-knot nematode disease and provided theoretical basis to control it, the author investigated the condition of root-knot nematode disease of Angelica dahurica in Nanchuan from 2012 to 2014. Five-sampling method was used to collect soil and Angelica dahurica with root-knot nematode disease in different periods, sucrose centrifugal flotation method was used to separate root-knot nematodes. The results showed that the root-knot nematode disease in loam, sandy and sandy loam soil was more serious than that in clay soil. Meanwhile, disease occurrence in continuous cropping land was more serious than that in intercropping and crop rotation land. The density of second-stage juveniles of nematodes increased from 71 individuals/1000 g dry soil in January to 1615 individuals/1000 g dry soil in October, and March was the peak period. Nematodes centralized around the root in horizontal level, and decreased with the increase of depth.