Study on Leaf Phenotype, Soluble Sugar and Chlorophyll Fluorescence Characteristics Response to Heat Stress in‘Jumeigui’Grapes

doi:10.11924/j.issn.1000-6850.casb15030218

Abstract

Abstract: The response to heat stress in‘Jumeigui’grapes (Vitis vinifera L.×V. labrusca L.) has been studied, to provide the warning and measures in management of facility cultivation. Annual giant‘Jumeigui’grapes were selected and the changes of indexes, including leaf phenotype and organization structure, the content of soluble sugar and chlorophyll fluorescence, were measured under different treatments (27, 32, 37, 42℃). The results showed that under treatment of 37℃ , the leaf veins of‘Jumeigui’grapes appeared water loss phenomenon. At 42℃, the leaf started to dry up, the young leaves did not unfolding and the plants eventually died. At 27, 37℃ there were no changes of leaf mesophyll tissue, and the palisade tissue was orderly arranged. But under the condition of 42℃, mesophyll tissue showed water loss to shrink. And at 42℃, the content of soluble sugar increased obviously, the O-I phase value of photosynthesis OJIP curve in original reactions and ABS/RC, ETo/RC, TRo/RC, DTo/RC maintained a relative high level. The conclusion provides the idea that the leaf phenotype, the content of soluble sugar and chlorophyll fluorescence indicators of‘Jumeigui’grapes are related to the processing degree and processing time of high temperature conditions. Under the treatment of 42℃,‘Jumeigui’grapes show the irreversible damage at 22 d.

References

[1] Wahid A,Gelani S,Ashraf M, et al. Heat tolerance in plants:An overview [J].Environmental and Experimental Botany,2007,61(3):199-223.
[2] 王涛,田雪瑶,谢寅峰,等.植物耐热性研究进展[J].南农业大学学报,2013,28(5):719-726.
[3] Strasser R J. Measuring fast fluorescence transients to address environmental questions:the OJIP test [J].Photosynthesis:from light to biosphere,1995,5:977-980.
[4] 冯建灿,胡秀丽,毛训甲.叶绿素荧光动力学在研究植物逆境生理中的应用[J].经济林研究,2002,20(4):14-18.
[5] 杨治元.无核精品巨玫瑰葡萄数字化栽培技术[J].中国南方果树,2011,40(3):98-100.
[6] 李为福,何建军,谢兆森,等.外源脱落酸对‘巨玫瑰’葡萄着色及浆果品质的影响[J].中国南方果树,2012,41(1):22-26.
[7] 李峰.植物石蜡切片制作(paraffin section)的试验流程探讨[J].科技信息,2012 (5): 104-105.
[8] 邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000.
[9] 胡学华,蒲光兰,赖建权,等.核桃营养枝与结果枝叶绿素荧光动力学的特性比较[J].北方园艺,2010(6):35-37.
[10] Mahoney J M,Rood S B. Response of a hybrid poplar to water table decline in different substrates [J].Forest Ecology and Management,1992,54(1):141-156.
[11] 陈建明,俞晓平,程家安.叶绿素荧光动力学及其在植物抗逆生理研究中的应用[J].浙江农业学报,2006,18(1):51-55.
[12] 孙猛,吕德国,刘威生.不同品种群15个杏品种荧光特性研究初探[J].中国农学通报,2011,27(6):171-176.
[13] 胡良平,高辉.如何正确运用单因素多水平设计定量资料方差分析[J]. 西医结合学报,2008,6(3):316-319.
[14] 陈贻竹,李晓萍,夏丽,等.叶绿素荧光技术在植物环境胁迫研究中的应用[J].热带亚热带植物学报,1995,3(4):79-86.
[15] 李艳艳,李植良,黎振兴,等.热胁迫对不同耐热性茄子叶片有关生理指标及组织结构的影响[Z].园艺学报,2011,38:2553.
[16] 陈雄伟,梁广坚,周丽萍,等.人工高温下苋菜叶的抗热特征和光合性能[J].热带亚热带植物学报,2012,20(2):149-155.
[17] 刘媛媛,滕中华,王三根,等.高温胁迫对水稻可溶性糖及膜保护酶的影响研究[J].西南大学学报:自然科学版,2008,30(2):59-63.
[18] 杨军,刘向蕊,陈小荣,等.双季早稻淦鑫203乳熟初期高温处理后叶片某些生理特性的动态变化[J].江西农业大学学报,2012,34(5):878-885.
[19] Baker N R. Chlorophyll fluorescence:a probe of photosynthesis in vivo [J].Annual Review of Plant Biology,2008,59:89-113.
[20] 李钦夫,李征明,纪建伟,等.叶绿素荧光动力学及在植物抗逆生理研究中的应用[J].湖北农业科学,2013,52(22):5399-5402.
[21] 孙艳,徐伟君,范爱丽.高温强光下水杨酸对黄瓜叶片叶绿素荧光和叶黄素循环的影响关[J].应用生态学报,2006,17(3):399-402.
[22] 郑秋玲,谭伟,马宁,等.钙对高温下‘巨峰’葡萄叶片光合作用和叶绿素荧光的影响[J].中国农业科学,2010,43(9):1963-1968.
[23] 郭延,平一,周慧芬,等. 高温胁迫对柑橘光合速率和光系统Ⅱ活性的影响[J].应用生态学报,2003,14(6):867-870.
[24] Cendrero mateo M P, Do carom silva A E, Salvucci M, et al. Steady state chlorophyll fluorescence (Fs) as a tool to monitor plant heat and drought stress[C].GU Fall Meeting Abstracts,2012,1:0421.
[25]李鹏民,高辉远,RetoSJ.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J].植物生理与分子生物学学报,2005,31(6):559-566.
[26] Strasser R J, Tsimilli M,Srivastava A. Analysis of the chlorophyll a fluorescence transient[M].Springer Netherlands,2004:321-362.