Study on Six Species of Beans by Fourier Transform Infrared Spectroscopy Based on Curve-fitting

doi:10.11924/j.issn.1000-6850.casb15070043

Abstract

Abstract: In order to identify different kinds of beans quickly, provide the basis to extract the beans protein and so on, 54 samples were studied by Fourier Transform Infrared (FTIR) spectroscopy combined with hierarchical cluster analysis (HCA) and curve-fitting analysis. The FTIR results showed that the infrared spectra of the six kinds of beans were similar, but significant differences in wave-numbers and absorption intensities of peaks were observed in the range of 1800-1000 cm^-1. The second derivative spectra in the range of 1800-1000 cm^-1 were selected to perform hierarchical cluster analysis (HCA) and yielded 100% accuracy. The amide I band (1700-1600 cm^-1) in the original spectra was used for decomposition and curve fitting to evaluate the contents of secondary structure of protein. The results showed the amide Ⅰ regions comprised nine sub-bands in the infrared absorbance spectra of beans. The results showed the contents of α-helix, β-turn, unordered structure and β-sheet were dissimilar in the six kinds of beans. It is proved that the secondary structure of protein was different in the six kinds of beans. FTIR spectroscopy combined with curve-fitting analysis could be used to discriminate different kinds of beans rapidly and accurately and provide valuable information about the protein secondary structures of beans.

CLC Number:

O657.3

Li Huimei,Liu Gang,Ma Dianxu,Ou Quanhong,Yu Haichao and Liu Yan. Study on Six Species of Beans by Fourier Transform Infrared Spectroscopy Based on Curve-fitting[J]. Chinese Agricultural Science Bulletin, 2015, 31(32): 81-86.

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