Simultaneous Determination of 11 Trace Elements in Cymbopogon citratus by ICP-OES with Microwave Digestion

doi:10.11924/j.issn.1000-6850.casb19010128

Abstract

Abstract:

In this study, a method was developed for the first time for simultaneous determination of 11 trace elements (Zn, Cd, Pb, Co, Ni, B, Mn, Fe, Cr, Mg, Cu) in Cymbopogon citratus by using inductively coupled plasma optical emission spectrometer (ICP-OES) with microwave digestion. Samples were digested completely by using HNO₃-H₂O₂ mixed acid. The instrumental working parameters and microwave digestion process were optimized. The results showed that: the linear relationship of 11 trace elements was good, the correlative coefficients were in the range of 0.9983-0.9999, the limits of detection (LOD) were in the range of 0.000050-0.081325 mg/L, the limits of quantification (LOQ) were in the range of 0.0002-0.2455 mg/L, the recovery rates were in the range of 90.00%-102.50%, and the relative standard deviations (RSD, n=6) were in the range of 0.25%-1.64%; the concentration of the 11 trace elements in Cymbopogon citratus from Yuanmou and Longchuan had significant differences, the order of was Mg>Fe>Zn>Mn>Cu>Ni>B, and Cd, Pb, Cr and Co were not detected; the concentration of the same element was also different among the samples from different sources. This method has the advantages of rapidness, simplicity, accuracy, high sensitivity and good stability, which is suitable for simultaneous determination of various elements in Cymbopogon citratus.

Key words: microwave digestion, ICP-OES, Cymbopogon citratus, determination, trace elements

CLC Number:

O657.3

Yuan Jianmin, He Lu, Yang Xiaoqiong, Xu Zhiping, Kong Weixi. Simultaneous Determination of 11 Trace Elements in Cymbopogon citratus by ICP-OES with Microwave Digestion[J]. Chinese Agricultural Science Bulletin, 2020, 36(14): 69-73.

Figures/Tables 3

References 26

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元素	检测波长/nm	BEC/(mg/L)	回归方程	相关系数r	检出限LOD/(mg/L)	定量下限LOQ/(mg/L)	RSD/%
Zn	206.200	0.0103009	y=32659.568+754274.11x	0.9997	0.021223	0.0625	1.55
Cd	214.441	0.0067566	y=14483.256+924794.32x	0.9999	0.000050	0.0002	0.31
Pb	220.353	0.1564736	y=1732.4260+2517.224x	0.9999	0.001240	0.0035	0.32
Co	228.615	0.0384767	y=7915.1250+323620.31x	0.9997	0.000152	0.0006	0.20
Ni	231.604	0.0442303	y=20165.023+428508.18x	0.9995	0.003403	0.0100	0.76
B	249.773	0.0567235	y=1349.261+410065.26x	0.9993	0.003325	0.0100	0.84
Mn	257.610	0.0067338	y=131035.44+3870136.0x	0.9999	0.002845	0.0090	0.60
Fe	259.940	0.0243424	y=67485.268+537353.25x	0.9993	0.039154	0.1153	0.85
Cr	267.716	0.3562215	y=97056.226+606.08561x	0.9999	0.024625	0.0735	1.08
Mg	279.553	0.0023844	y=1124979+27451020x	0.9983	0.081325	0.2455	1.05
Cu	324.754	0.0931254	y=31598.023+881367.53x	0.9999	0.010245	0.0332	1.02

元素	检测波长/nm	BEC/(mg/L)	回归方程	相关系数r	检出限LOD/(mg/L)	定量下限LOQ/(mg/L)	RSD/%
Zn	206.200	0.0103009	y=32659.568+754274.11x	0.9997	0.021223	0.0625	1.55
Cd	214.441	0.0067566	y=14483.256+924794.32x	0.9999	0.000050	0.0002	0.31
Pb	220.353	0.1564736	y=1732.4260+2517.224x	0.9999	0.001240	0.0035	0.32
Co	228.615	0.0384767	y=7915.1250+323620.31x	0.9997	0.000152	0.0006	0.20
Ni	231.604	0.0442303	y=20165.023+428508.18x	0.9995	0.003403	0.0100	0.76
B	249.773	0.0567235	y=1349.261+410065.26x	0.9993	0.003325	0.0100	0.84
Mn	257.610	0.0067338	y=131035.44+3870136.0x	0.9999	0.002845	0.0090	0.60
Fe	259.940	0.0243424	y=67485.268+537353.25x	0.9993	0.039154	0.1153	0.85
Cr	267.716	0.3562215	y=97056.226+606.08561x	0.9999	0.024625	0.0735	1.08
Mg	279.553	0.0023844	y=1124979+27451020x	0.9983	0.081325	0.2455	1.05
Cu	324.754	0.0931254	y=31598.023+881367.53x	0.9999	0.010245	0.0332	1.02

元素	元谋香茅草/(mg/kg)	陇川香茅草/(mg/kg)	RSD/%
Zn	65.73c	80.32c	0.50
Cd	ND	ND	0.19
Pb	ND	ND	0.38
Co	ND	ND	0.49
Ni	1.68f	1.25f	3.42
B	0.74g	0.65g	2.85
Mn	28.20d	37.32d	0.98
Fe	158.40b	185.30b	0.72
Cr	ND	ND	0.36
Mg	524.40a	582.36a	0.03
Cu	10.04e	11.35e	1.42

元素	元谋香茅草/(mg/kg)	陇川香茅草/(mg/kg)	RSD/%
Zn	65.73c	80.32c	0.50
Cd	ND	ND	0.19
Pb	ND	ND	0.38
Co	ND	ND	0.49
Ni	1.68f	1.25f	3.42
B	0.74g	0.65g	2.85
Mn	28.20d	37.32d	0.98
Fe	158.40b	185.30b	0.72
Cr	ND	ND	0.36
Mg	524.40a	582.36a	0.03
Cu	10.04e	11.35e	1.42

元素	样品浓度/(mg/L)	样品溶质质量/(μg)	加标值/(μg)	加标后测定值/(mg/L)	加标后溶质质量/(μg)	平均回收率/%	RSD/%
Zn	0.26	1.30	1.0	0.4500	2.2500	95.00	1.64
Cd	0.00	0.00	1.0	0.1870	0.9350	93.50	0.25
Pb	0.00	0.00	1.0	0.1830	0.9150	91.50	1.35
Co	0.00	0.00	1.0	0.1850	0.9250	92.50	0.78
Ni	0.01	0.05	1.0	0.1995	0.9975	94.75	1.26
B	0.00	0.00	1.0	0.1923	0.9615	96.15	1.35
Mn	0.11	0.55	1.0	0.2985	1.4925	94.25	1.21
Fe	0.63	3.15	1.0	0.8288	4.1440	99.40	1.33
Cr	0.00	0.00	1.0	0.1800	0.9000	90.00	1.25
Mg	2.10	10.50	1.0	2.2850	11.4250	92.50	0.76
Cu	0.04	0.20	1.0	0.2450	1.2250	102.50	0.25