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Chinese Agricultural Science Bulletin ›› 2022, Vol. 38 ›› Issue (14): 53-59.doi: 10.11924/j.issn.1000-6850.casb2021-0570

Special Issue: 生物技术； 园艺

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Preliminary Study on the Active Substances of Different Medicinal Parts of Daphne tangutica Maxim of Different Planting Years

ZHOU Zhiming1(), LV Biao1,4(), YAN Fang2,4, ZHANG Chunyun3, WANG Haiping3, WANG Qinli2, WANG Jiandong3, XU Tao2, ZHANG Chunmei2,3, CHEN Ye2   

  1. 1College of Resources and Environment, Gansu Agricultural University, Lanzhou 730000
    2Applied Technology Research Institute on Original Plants for Zushima of Hexi University, Zhangye, Gansu 734000
    3Taikang Pharmaceutical Limited Liability Company of Gansu Province, Wuwei, Gansu 733000
    4Research Institute for Rural Revitalization of Hexi University, Zhangye, Gansu 734000
  • Received:2021-05-31 Revised:2021-09-16 Online:2022-05-15 Published:2022-06-07
  • Contact: LV Biao E-mail:969709419@qq.com;lvbiao6123@163.com

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Abstract:

This study is to analyze the contents of three components and their changes in the rhizomes and stems of wild Daphne tangutica Maxim grown in different regions and of different planting years. High performance liquid chromatography (HPLC) and ZORBAX SB-C18 (4.6 mm ×150 mm) chromatographic column were used. The mobile phases of acetonitrile: 0.05% phosphoric acid (10:90) and methanol: water (20:80) were adopted. The flow rate was 0.9 mL/min, and the detection wavelength was 265 nm and 327 nm, respectively. The temperature was 40℃, and the sample load was 20 μL. The established HPLC system had good linear range (R2 > 0.999), the relative standard deviation (RSD) of precision, stability and repeatability were all less than 2%, and the recovery rate was 95.10%-95.84%. The content analysis showed that the content of syringin in the same part increased significantly in the fifth year (P< 0.05); the content of Daphentin and 7-hydroxycoumarin in the root bark and stem bark in Huzhu area and the stem bark in Haxi area decreased significantly in the fifth year (P< 0.05). The order of Daphentin and 7-hydroxycoumarin content in stem bark in Huzhu area and root bark in Haxi area was 5-year-old > 8-year-old > 3-year-old and 5-year-old > 3-year-old > 8-year-old, respectively (P< 0.05). For the same year samples, except that the content of Daphentin, 7-hydroxycoumarin and syringin in stem bark were significantly higher than those in root bark (P< 0.05), the content of Daphentin in stem bark in Haxi area was significantly higher than that in root bark and stem bark in Huzhu area (P< 0.05). The content of Daphentin of 5-year-old and 8-year-old and the content of 7-hydroxycoumarin and syringin of 3-year-old were in the order of Huzhu stem bark > Haxi stem bark > Huzhu root bark > Haxi root bark (P< 0.05); the content of syringin of 5-year-old and 8-year-old were in the order of Huzhu stem bark > Huzhu root bark > Haxi stem bark > Haxi root bark (P< 0.05). The content of most components in the same part of Daphne tangutica Maxim were higher in the fifth year; and the content of most components in stem bark was higher than that in root bark in the same year plant; the content of components in the stem bark of 5-year-old Daphne tangutica Maxim in Haxi area was the highest.

Key words: Daphne tangutica Maxim, under different conditions, high performance liquid chromatography, content determination

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