Microbial Community Dynamics During the Sludge Thermophilic Composting Process

doi:10.11924/j.issn.1000-6850.casb15060130

Abstract

Abstract: The aims of this study was to illustrate the dynamic changes of bacterial community during the process of municipal sludge composting, and provided theoretical basis and technical support to accelerate the compost process. The bacterial community structures and dynamic changes were analyzed by the culture-dependent method (plate count method) and culture-independent methods (denaturing gradient gel electrophoresis, and high throughput sequencing) during the municipal sludge composting process. The mesophilic bacteria (28℃) were firstly decreased and got increased gradually with the compost process. Instead, thermophilic bacteria (55℃) were declined after stimulation at the beginning of compost. Six mesophilic bacteria (Paracoccus thiocyanatus, Advenella sp., Arthrobacter sp., Lysobacter sp., Serratia sp. and Rhodococcus pyridinivorans) and 2 thermophilic bacteira (Ureibacillus thermosphaericus and Geobacillus thermodenitrificans) were isolated by the culture-dependent method. During the composting process, bacteria diversity changed obviously resulted by the DGGE fingerprint and 4 branches were divided by the cluster analysis. There were 6 phyla, 8 classes of the predominant bacteria were gained by the high throughput sequencing (the 7th day of the composting), and 88.6% of the bacteria distributed in Sphaerobacte order, Thermomicrobia class, Chloroflexi phylum. The results of this study revealed that bacteria belonged to Sphaerobacte order were predominant in the amount and diversity in the composting.

CLC Number:

S141.4

References

[1] 丁文川,李宏,郝以琼,等.污泥好氧堆肥主要微生物类群及其生态规律[J].重庆大学学报(自然科学版),2002,25(6): 113-116.
[2] 王伟东,王小芬,朴哲,等.堆肥化过程中微生物群落的动态[J].环境科学,2007,28(11): 2591-2597.
[3] 黄得扬, 陆文静, 王洪涛. 有机固体废物堆肥化处理的微生物学机理研究[J]. 环境工程学报, 2004, 5(1): 12-18.
[4] Hassen A, Belguith K, Jedidi N, et al. Microbial characterization during composting of municipal solid waste [J]. Bioresource Technology, 2001, 80: 217-225.
[5] 王春铭,卢建文,雷恒毅,等.城市污泥堆肥过程中微生物研究[J]. 生态环境, 2007, 16(2): 462-466.
[6] 何云晓,何丹.微生物在污泥堆肥处理中的优化改良[J]. 安全与环境工程, 2013, 20(4): 51-54.
[7] Whiteley S, Bailey J. Bacterial community structure and physiological state within an industrial phenol bioremediation system [J]. Applied and Environmental Microbiology, 2000, 66(6):2400-2407.
[8] Amann I, Ludwig W, Schleifer H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation [J]. Microbiological Review, 1995, 59(1):143-169.
[9] Muyzer G, de Waal C, Uitterlinden G. Profiling of complex microbial population by denaturing gradient gel electrophoresis analysis of polymerase chain reaction amplified genes encoding for 16S rRNA [J]. Applied and Environmental Microbiology, 1993, 59(3):695-700.
[10] 赵斌,何绍江. 微生物学实验[M].北京:科学出版社,2002. 85-86.
[11]韩亚飞,伊文慧,王文波,等.基于高通量测序技术的连作杨树人工林土壤细菌多样性研究[J].山东大学学报(理学版),2014,49(5): 1-6.
[12] Li A, Yang S, Li X, et al. Microbial population dynamics during aerobic sludge granulation at different organic loading rates [J]. Water Research, 2008, 42 (13), 3552-3560.
[13] Zhang J, Zeng G, Chen Y, et al. Effects of physico-chemical parameters on the bacterial and fungal communities during agricultural waste composting [J]. Bioresource Technology, 2011, 102 (3), 2950-2956.
[14] 刘善江,陈桂梅,夏雪,等.污泥堆肥过程中的理化性质及微生物学特性[J].水土保持学报,2011,25(5): 89-93.
[15] Blanc M, Marilley L, Beffa T, et al. Thermophilic bacterial communities in hot composts as revealed by most probable number counts and molecular (16S rDNA) methods [J]. FEMS Microbiology Ecology, 1999, 28 (2): 141-149.
[16] Dees P, Ghiorse W. Microbial diversity in hot synthetic compost as revealed by PCR-amplified rRNA sequences from cultivated isolates and extracted DNA [J]. FEMS Microbiology Ecology, 2001, 35 (2): 207-216.
[17] Peters S, Koschinsky S, Schwieger F, et al. Succession of microbial communities during hot composting as detected by PCR single-strand-conformation polymorphism-based genetic profiles of small subunit rRNA genes [J]. Applied and Environmental Microbiology, 2000, 66 (3): 930-936.
[18] Watanabe K, Nagao N, Toda T, et al. Bacterial community in the personal-use composting reactor revealed by isolation and cultivation-independent method[J]. Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Wastes, 2010, 45(5): 372-378.
[19] Garcia R., Cuesta G, Sanchis E, et al. Bacteria involved in sulfur amendment oxidation and acidification processes of alkaline 'alperujo' compost[J]. Bioresource Technology, 2011, 102(2): 1481-1488.
[20] Papasotiriou F, Varypatakis K, Christofi N, et al. Olive mill wastes: A source of resistance for plants against Verticillium dahliae and a reservoir of biocontrol agents [J]. Biological Control, 2013, 67(1): 51-60.
[21] Hayat R, Sheirdil R, Iftikhar M, et al. Characterization and identification of compost bacteria based on 16S rRNA gene sequencing [J]. Annals of Microbiology, 2013, 63(3): 905-912.
[22] Sebok F, Dobolyi C, Cserhati M, et al. Survival of Alkane-degrading microorganisms in biogas digestate compost in microcosm experiments [J]. Applied Ecology and Environmental Research, 2014, 12(4): 947-958.
[23] Pirog T, Konon A, Sofilkanich A, et al. Effect of surface-active substances of Acinetobacter calcoaceticus IMV B-7241, Rhodococcus erythropolis IMV Ac-5017, and Nocardia vaccinii K-8 on phytopathogenic bacteria[J]. Applied Biochemistry and Microbiology, 2013, 49(4): 360-367.
[24] Zainudin M, Hassan M, Tokura M, et al. Indigenous cellulolytic and hemicellulolytic bacteria enhanced rapid co-composting of lignocellulose oil palm empty fruit bunch with palm oil mill effluent anaerobic sludge [J].Bioresource Technology, 2013, 147: 632-635.