Abstract: The paper aims to establish an analytical method for determining florasulam residues in soil. Soil samples with acetonitrile were shaking extraction, then went through the membrane after standing, measured by HPLC-PAD directly. The results showed that with the addition concentration of 1.0 and 10.0 mg/kg, the average recoveries of florasulam in black soil, red soil, paddy soil were 82.4%-86.6%, 83.0%-87.7% and 85.6%-85.9%; the coefficient of variation were 1.9%-2.3%, 0.6%-8.2% and 2.3%-3.4%. The detection limit of florasulam was 1.0×10-9 g, the quantification limit was 1.0 mg/kg. The method is simple and fast sample analysis. Acuracy, precision and sensitivity all meet the requirement of pesticide residues analysis, and can be used for the determining florasulam residue in soil.