Analysis of Blast Resistance and Genetic Background in Rice Restorer ‘Chenghui 177’

doi:10.11924/j.issn.1000-6850.2014-2356

Abstract

Abstract: In order to explore durable blast resistance and genetic basis of high combining ability in ‘Chenghui 177’, the resistant spectrum, blast resistance gene, and genetic background were analyzed using in vitro inoculation, gene sequencing and the polymorphism of SSR molecular markers. The resistance frequencies of ‘Chenghui 177’ against 63 isolates of rice blast and 16 differential isolates were 66.67% and 68.75%, respectively. It carried Pikm derived from Lemont which was a tropical japonica variety. The results of molecular genetic background analysis showed that, among 148 polymorphic SSR loci, ‘Chenghui 177’ had acquired respectively 70.95% and 20.95% from ‘Mianhui 502’ and Lemont. In the genome of ‘Chenghui 177’, 64% to 100% of the polymorphic SSR loci on chromosome 1-10 were derived from ‘Mianhui 502’, while Lemont was responsible for respectively 77.78% and 60.00% of the polymorphic SSR loci on chromosome 11 and 12 in ‘Chenghui 177’. The results suggested that ‘Chenghui 177’ successfully pyramided stable blast resistance of Lemont and high combining ability of ‘Mianhui 502’.

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