Abstract: Harmonia axyridis is a common natural enemy to farmland pests. This study aims at the effect of light wave, light source distance and temperature on the phototaxis behavior of Harmonia axyridis adult, and provide theoretical basis for designing insecticidal lamp which could avoid harming to natural enemies of pests. Sixteen kinds of monochromatic light waves and two kinds of fixed control light waves were used. Harmonia axyridis of different ages and sexes were placed in three light boxes with different light source distances, but the humidity and temperature were kept stable. Then phototaxis rates of Harmonia axyridis under different light waves and light source distances were tested. Besides, light tendency of Harmonia axyridis under different temperatures was also studied. According to the results, the favorite light waves of Harmonia axyridis adults were at 500, 605 and 550 nm and the repellent light waves were at 473 and 407 nm. The phototaxis response rate at the distance of 0.65 m away from the light source was significantly higher than that of 1.15 and 2.15 m. Too high and too low temperature had certain inhibitory effect on light tendency of Harmonia axyridis. In conclusion, phototaxis behavior of Harmonia axyridis adult was obviously influenced by its selection of light wave, light source distance and temperature.