Bioinformatic Analysis of Toxin Protein Gene hipA from Haemophilus parasuis

doi:10.11924/j.issn.1000-6850.casb15070129

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (2): 1-4.doi: 10.11924/j.issn.1000-6850.casb15070129

Special Issue: 生物技术

Bioinformatic Analysis of Toxin Protein Gene hipA from Haemophilus parasuis

Che Yongliang, Chen Rujing, Wang Longbai, Wu Xuemin, Liu Yutao, Wang Chenyan, Zhou Lunjiang

(Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences/Fujian Animal Diseases Control Technology Development Center, Fuzhou 350013)

Received:2015-07-22 Revised:2015-11-26 Accepted:2015-12-03 Online:2016-01-28 Published:2016-01-28

Abstract

Abstract: The aim of this study was to predict secondary structure and B-cell epitopes of toxin protein HipA of Haemophilus parasuis. The hipA gene sequence was searched from GenBank. By using bioinformatics methods, the physicochemical property, the secondary structure and B-cell epitope of HipA protein were predicted. The results showed that the IP of HipA was 7.23 and the first 50 amino acids was signal peptide. Using the SOPMA method, prediction of the secondary structure of the HipA showed that α-helix, random coils and extended strand were the main structural types of the flexible region in secondary structure, and there was a small amount of β-turn. The HipA protein was supposed to contain 17 potential antigen epitopes. These results provided a theoretical basis for a further study of the toxin protein (HipA) from Haemophilus parasuis.

CLC Number:

S852.612

Che Yongliang,Chen Rujing,Wang Longbai,Wu Xuemin,Liu Yutao,Wang Chenyan and Zhou Lunjiang. Bioinformatic Analysis of Toxin Protein Gene hipA from Haemophilus parasuis[J]. Chinese Agricultural Science Bulletin, 2016, 32(2): 1-4.

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Bioinformatic Analysis of Toxin Protein Gene hipA from Haemophilus parasuis

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