Prokaryotic Expression of Bacteriophage Lysin of Riemerella anatipestifer

doi:10.11924/j.issn.1000-6850.casb15070112

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (2): 5-8.doi: 10.11924/j.issn.1000-6850.casb15070112

Special Issue: 生物技术；畜牧兽医

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Prokaryotic Expression of Bacteriophage Lysin of Riemerella anatipestifer

Cheng Longfei, Fu Qiuling, Chen Hongmei, Fu Guanghua, Shi Shaohua, Wan Chunhe, Huang Yu

(Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013)

Received:2015-07-21 Revised:2015-12-23 Accepted:2015-10-23 Online:2016-01-28 Published:2016-01-28

Abstract

Abstract: The objective of this test was to make a prokaryotic express of the bacteriophage RAP44 lysin gene of Riemerella anatipestifer. The gene of lyase was amplified by PCR using genomic DNA of bacteriophage RAP44 and inserted into pGEX-6P-1 vector. The constructed recombinant plasmid was transformed into Escherichia coli BL21(DE3) to express fusion protein, which was purified by Glutathione-Sepharose 4B. The results showed that expression plasmid pGEX-N was constructed successfully. The soluble recombinant protein about 37 kD was expressed after induced by IPTG. The purified fusion protein was generated in this study, which laid a foundation for the functional study of bacteriophage lysin of Riemerella anatipestifer.

CLC Number:

S855.1

Cheng Longfei,Fu Qiuling,Chen Hongmei,Fu Guanghua,Shi Shaohua,Wan Chunhe and Huang Yu. Prokaryotic Expression of Bacteriophage Lysin of Riemerella anatipestifer[J]. Chinese Agricultural Science Bulletin, 2016, 32(2): 5-8.

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Prokaryotic Expression of Bacteriophage Lysin of Riemerella anatipestifer

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