Effects of Different Treatments on Honeybee Gathering Soybean Pollen

doi:10.11924/j.issn.1000-6850.casb15090050

Abstract

Abstract: To increase the visiting enthusiasm of honeybee to soybean flowers and improve the podding rate of hybrid soybean, hanging brood pheromone, feeding 8-Br-cGMP and soy flower syrup in nest were used to treat bee colony, the control group was feeding syrup (1:1). By gathering pollen, video observation and quantitative real-time PCR, soybean pollen weight percentage, bee percentage of collecting soybean pollen and relative quantitative expression of Amfor and Ammvl mRNA of the three methods were compared. The results showed that the concentration of 400 LEqu brood pheromone and 0.5 mg/L 8-Br-cGMP was selected, and soy flower syrup was used to deal with the colony. The soybean pollen weight percentage of the control group was 3.05%, and that of the three treatments was respectively 10.42%, 8.53% and 5.99%. 400 LEqu and 0.5 mg/L 8-Br-cGMP were significantly different from the control group (P < 0.05). Honeybees proportions on soybean flowers among foraging pollen honeybees of the control group was 55.09%, and those of the three treatments were higher than that. There was no significant difference between the treatment groups and control group (P > 0.05). The three kinds of treatments can improve the visiting enthusiasm of the honeybees to soybean flowers and the number of honeybee who collect the soybean pollen, thus increase the visiting probability on soybean flowers.

CLC Number:

S897

Ma Weihua,Shen Jinshan,Wu Wenqing,Song Huailei,Song Zhuoqin,Zhang Xufeng and Shao Youquan. Effects of Different Treatments on Honeybee Gathering Soybean Pollen[J]. Chinese Agricultural Science Bulletin, 2016, 32(2): 9-14.

References

[1] 杨桂华,李建平,李茂海.三种传粉昆虫在网室内对大豆不育系传粉行为研究初报[A].当代昆虫学研究-中国昆虫学会成立60周年纪念大会暨学术讨论会论文集[C].北京:中国农业科学技术出版社,2004.
[2] 赵丽梅,孙寰,马春森,等.大豆昆虫传粉研究初探[J].大豆科学,1999(1):73-76.
[3] 谢甫绨.大豆雄性不育及杂种优势利用研究进展[J].沈阳农业大学学报,2008,39(2):131-136.
[4] 孙寰,张井勇,王玉民,等.木豆,苜蓿和大豆3种豆科作物杂种优势利用概述[J].中国农业科学,2009, 42(5):1528-1539.
[5] Robacker D C, Flottum R K, Sammataro D, et al. Effects of climatic and edaphic factors on soybean flowers and on the subsequent attractiveness of the plants to honey bees[J].Field Crops Research,1983,6:267-278.
[6] Koelling P D, Kenworthy W J, Caron D M. Pollination of male- sterile soybean in caged plots [J]. Crop Science, 1981, 21:551-559.
[7] 历延芳,赵丽梅.蜜蜂为杂交大豆授粉技术研究获得创新性成果[J].中国蜂业,2014, 65(2):13.
[8] Robinson G E. Genomics and integrative analyses of division of labor in honeybee colonies [J]. American Naturalist, 2002, 160: S160-S172.
[9] Ben-shahar Y, Dudek N L. Phenotypicde constraction reveals involvement of manganese transporter malvolio in honey bee division of labor [J]. Journal of Experiment al Biology, 2004, 207: 3281-3288.
[10] Heylen K, Gobin B, Billen J, et al. Amfor expression in the honeybee brain: a trigger mechanism for nurse–forager transition [J].Journal of Insect Physiology, 2008, 54:1400-1403.
[11] Toth A L, Robinson G E. Evo-devo and the evolution of social behavior: brain gene expression analyses in social insects [J].Cold?Spring?Harbor symposia on quantitative?biology, 2009, 74: 419-426.
[12] 孟娇,马卫华,赵慧婷,等.中华蜜蜂Malvolio基因 Acmvl 的克隆及组织表达分析[J].昆虫学报, 2015, 58(7): 721-730.
[13] Scharlaken B, Graaf D C, Goossens K, et al. Reference gene selection for insect expression studies using quantitative real-time PCR: The head of the honeybee, Apis mellifera, after a bacterial challenge [J]. Journal of Insect Science, 2008, 8:1-10.
[14] Pakiw T. Brood pheromone regulates foraging activity of honey bees (Hymenoptera: Apidae) [J]. Journal of Economic Entomology, 2004, 97(3): 748-751.
[15] 张晗译.幼虫信息素对西方蜜蜂采集行为的影响[J].中国蜂业,2008,59(9):49.
[16] 丁桂玲译. 幼虫信息素与蜜蜂的采集行为[J].中国蜂业,2011,62(10):48.
[17] Alaux C, Le Conte Y, Adams H A, et al. Regulation of brain gene expression in honey bees by brood pheromone [J].Genes, Brain and Behavior ,2009,8: 309-319.
[18] Le Conte Y, Mohammedi A, Robinson G E. Primer effects of a brood pheromone on worker honeybee behavioral development[J].Proceedings of the Royal Society B, 2001, 268: 163-168.
[19] Robinson G E. Genomics and integrative analyses of division of labor in honeybee colonies [J]. American Naturalist, 2002, 160: S160-S172.
[20] Ben-Shahar Y, Robichon A, Sokolowski M B, et al. Influence of gene action across different time scales on behavior [J]. Science, 2002, 296:741-744.