Abstract: The aims were to further research the characteristics of two-line nuclear sterile line ‘Ming S’, and obtain high-yielding combinations and get extensive application in the production. ‘Pei’ai 64S’ was used as the control, the botany characteristics and biological characteristics of ‘Ming S’ and ‘Pei’ai 64S’ were analyzed under the same cultivation and management conditions. Results showed that ‘Ming S’ had a good plant type, strong tillering ability, high effective panicles rate, strong resistance to blast, etc. The effective panicle number per plant was 17. Microscopic examination suggested that the pollen sterility had non-pollen type and a few typical failure plants. Its fertility rate and sterile degree were both 100%, selfing seed setting rate was 0. ‘Ming S’ had high stigma exsertion rate of 84.7% and allogamous seed setting rate was more than 70%. It had better rice quality which brown rice rate, head rice rate, gel consistency and alkali spreading value reached the standard level 1. It had resistance to rice blast, bacterial leaf blight and lower rates of sheath blight disease. Consequently, two-line nuclear sterile lines ‘Ming S’ possessed strong tillering characteristics, and it was thermo sensitive genic male sterile line with resistance to rice blast.