Abstract: In order to take recycling use of straw resources in Beijing area, which were applied in the field of Agaricus bisporus cultivation as productive resources. Based on traditional wheat straw formulation, corn stalks and mushroom residue were respectively added to instead of part of the wheat straw. Physical and chemical properties of the Agaricus bisporus compost in three different formulations during cultivation stage were determined by dry weighing method, kjeldahl method，burning weight method and so on, and bacterial communities characteristics were determined by DGGE analysis. After adding corn stalks and mushroom residue, the moisture content and carbon content of compost increased. The higher nitrogen content compost consists of, the higher yield would be. Different formulations yield followed the traditional wheat straw recipe, corn stalks recipe and mushroom recipe from higher to lower. Thermomonospora sp., Bacillus sp. and Microvirga sp. were the dominant microflora of the different formulations during cultivation stage. An uncultured bacterium (b13) which was an absolutely dominant microflora in the pinning phase may be relative to the formations of fungus-bud. The traditional wheat straw formulation had good physical and chemical properties and stability of bacteria communities, and the other two formulations which use corn stalks and mushroom residue to instead of part of the wheat straw respectively still need to be further optimized.