Abstract: The purpose was to study the optimum composition ratio of compound substrate with spent mushroom compound, peat, vermiculite, and perlite. With internationally best formula substrate (peat: vermiculite=2:1) used for CK, spent mushroom compound and peat were matched according to different proportions to get different substrates, physical and chemical nutrient properties were measured and effects on growth and nutrient uptake were investigated. The results showed that the bulk density, porosity and the pH of the compound substrates were all in the ideal condition. Compared with control, compound substrate including spent mushroom compost had lower available nitrogen and higher potassium content. However, compound substrates with high ratio of spent mushroom compound would lead to lower chlorophyll content and production. Appropriate proportion of spent mushroom compound was suitable for cherry tomato planting. The most suitable substrate was treatment 1(peat: mushroom: vermiculite: perlite =3:3:3:1), which had the best fruit quality. Appropriate proportion of spent mushroom compound and peat could get equal yield and fruit quantity, moreover, reduce the production cost.