Abstract: To determine the protein requirement amount of juvenileAcrossocheilius hemispinus , fishmeal and soybean meal were used as protein source, fish oil, soybean oil,α -starch, cellulose and appropriate amount of vitamins and minerals were added into test feed. JuvenileA. Hemispinus [average weight (12.51±0.15) g] were raised for 56 days with feed protein level of 26%, 30%, 34%, 38%, 42% and 46%, the effects of different protein levels on the growth and digestive enzyme activities of juvenile A. hemispinus were analyzed by comparing weight gain, feed coefficient and intestinal digestive enzyme activities. The results showed that the maximal weight gain rate was observed in 42P group, and then was in 38P group, the difference between the two was not significant (P >0.05), the minimal weight gain rate was observed in 26P group. The feed coefficient first decreased then rose with the increase of protein level, the lowest group was 42P and then was 38P, but the difference was not significant (P >0.05). The activities of trypsin in the intestine showed a trend of first rising and then decreasing with the increase of protein level in the feed, the highest group was 38P and the difference was significant (P <0.05) from other groups. To sum up, the appropriate feed protein level for juvenile A. hemispinus is 38%.