Early Responsive Proteins in Tobacco Infected by Tobacco Mosaic Virus

doi:10.11924/j.issn.1000-6850.casb17090051

Abstract

Abstract: In order to improve the early warning and prevention system of tobacco mosaic disease, the early responsive proteins in tobacco infected by TMV were screened through detecting the proteome changes. [Methods] iTRAQ was used to analyze the proteome changes and the genes expression was detected with qPCR method. [Results] Proteomic analysis showed that there were 160 differentially expressed proteins, including 64 up-regulated and 96 down-regulated proteins. The functions of these differential proteins were related to redox balance, RNA decay, stress response, chloroplast structure and photosynthesis. qPCR results demonstrated that the change trend in mRNA expressions of these differential proteins was consistent with iTRAQ data. Tobacco mosaic disease could be effectively controlled by timely application of antiviral agents and plant resistance inducers, which induced the changes in expression of PR10, LHCB, POD, HSP90, 14-3-3 and AGO1. [Conclusion] Some early responsive proteins were identified in tobacco infected by TMV, which will be helpful for clarifying the pathogenesis mechanism of tobacco mosaic disease and screening new antiviral agents and plant resistance inducers.

CLC Number:

S435.72

郭尧, and . Early Responsive Proteins in Tobacco Infected by Tobacco Mosaic Virus[J]. Chinese Agricultural Science Bulletin, 2018, 34(9): 40-47.

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