QTL Mapping and Epistatic Effect Analysis of Ear Traits in Maize

doi:10.11924/j.issn.1000-6850.casb17100071

Abstract

Abstract: The study aims at clarify the genetic basis of ear length (EL ), ear diameter (ED ) and kernel depth (KD ) at molecular level in maize. The recombinant inbred lines (RIL) generated from a crossing between inbred lines Yu82 and Yu87- 1 were used as materials. The phenotype identification of multiple locations, the quantitative trait locus (QTL) mapping and epistatic effect analysis based on genetic linkage with SNP marker were conducted in this study. The results showed that 18 QTLs associated with the three traits were identified, and the interaction with the environment was insignificant. In these QTLs, qEL1-1 associated withEL was in chromosome 1, and qKD2-1, qKD2-2 associated withKD was in chromosome 2, they could explain 6.21%, 10.11%, and 8.90% of the phenotypic variation, respectively. The epistatic effect analysis showed three pairs of locus interactions with the effect of 1.23%-6.49%, and one pair interaction reached the significant level. This study provides important reference for map- based cloning related to key genes and molecular mark assisted breeding.

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