Water Stress: Effects on Protective Enzyme Activities and Osmotic Regulatory Substances of Paris polyphylla in Shaanxi

doi:10.11924/j.issn.1000-6850.casb20191200933

Abstract

Abstract:

To investigate the effects of water stress on the protective enzyme activity and osmotic regulatory substance content of Paris polyphylla in Shaanxi Province, the seedlings cultivated in substrate were used as experimental materials, the polyethylene glycol-6000 (PEG-6000) solutions with concentrations of 5%, 10% and 15% were used to simulate different water stress gradients, and the leaves of P. polyphylla were sampled at different processing stages to determine the protective enzyme activity and osmotic regulatory substance content. The results showed that, after the seedlings of P. polyphylla treated with different concentrations of PEG solutions, the activities of superoxide dismutase (SOD) and peroxidase (POD) decreased initially, followed by an increase, and then a decrease. The content of proline increased significantly after 2 h treatments, which was 2.61 times that of the control when treated with 15% PEG for 5 d. The soluble sugar content increased significantly after 12 h treatments, and remained at a high level during the later stage of treatments. Malondialdehyde (MDA) accumulated rapidly after different water stress treatments for 2 h, and the content of MDA was 229.9% higher than that of the control group on the 5^th day of 10% PEG treatment. P. polyphylla has a certain degree of drought resistance, and it can adapt to mild water stress by increasing the activity of protective enzymes and the content of osmotic regulatory substances.

Key words: Paris polyphylla, water stress, polyethylene glycol, protective enzyme activity, osmotic regulatory substances

CLC Number:

Li Bo, He Yihan, Wang Nan, Sun Xiaochun, Huang Wenjing, Bi Shijie, Song Zhongxing, Tang Zhishu. Water Stress: Effects on Protective Enzyme Activities and Osmotic Regulatory Substances of Paris polyphylla in Shaanxi[J]. Chinese Agricultural Science Bulletin, 2020, 36(17): 57-61.

Figures/Tables 5

References 25

[1]	郭兰萍, 吕朝耕, 王红阳 , 等. 中药生态农业与几种相关现代农业及GAP的关系[J]. 中国现代中药, 2018,20(10):1179-1188.
[2]	王文佳, 李爽, 马泽众 , 等. 水分胁迫对春大豆叶片保护酶活性及相对电导率的影响[J]. 中国农学通报, 2019,35(11):14-18.
[3]	王楠, 高静, 黄文静 , 等. 旱、盐胁迫下黄芪种子萌发及其对水杨酸的响应[J]. 草业科学, 2018,35(1):106-114.
[4]	吴建慧, 郭瑶, 崔艳桃 . 水分胁迫对绢毛委陵菜叶绿体超微结构及光合生理因子的影响[J]. 草业科学, 2012,29(3):434-439.
[5]	范苏鲁, 苑兆和, 冯立娟 , 等. 水分胁迫对大丽花光合作用、蒸腾和气孔导度的影响[J]. 中国农学通报, 2011,27(8):119-122.
[6]	刘艳, 蔡贵芳, 陈贵林 . 干旱胁迫对甘草幼苗活性氧代谢的影响[J]. 中国草地学报, 2012,34(5):93-98.
[7]	吴波, 张寿文, 高丹 , 等. 干旱胁迫及复水过程对吴茱萸扦插苗活性氧清除系统的影响[J]. 广州中医药大学学报, 2013,30(1):78-82.
[8]	Rahimi Y, Taleei A, Ranjbar M . Long-term water deficit modulates antioxidant capacity of peppermint (Mentha piperita L.)[J]. Scientia Horticulturae, 2018,237(7):36-43.
[9]	董伊晨, 刘悦秋 . 土壤水分对异株荨麻(Urtica dioica)保护酶和渗透调节物质的影响及其与叶片光合和生物量的相关性[J]. 生态学报, 2009,29(6):2845-2851.
[10]	王东清, 李国旗, 苏德喜 . 干旱胁迫对两种罗布麻渗透调节物质积累和保护酶活性的影响[J]. 干旱区资源与环境, 2012,26(12):177-181.
[11]	黄文静, 孙晓春, 李铂 , 等. 干旱胁迫诱导半夏倒苗的细胞程序性死亡研究[J]. 中国中药杂志, 2019,44(10):2020-2025.
[12]	梁宗锁, 杨东风, 杨宗岐 , 等. 不同土壤水分对丹参叶片总酚酸类成分积累及相关酶活性的影响[J]. 浙江理工大学学报, 2013,30(4):573-578.
[13]	Cheng L, Han M, Yang L M , et al. Changes in the physiological characteristics and baicalin biosynjournal metabolism of Scutellaria baicalensis Georgi under drought stress[J]. Industrial crops and products, 2018,122(10):473-482.
[14]	张宇, 周自云, 夏鹏国 , 等. 干旱胁迫对柴胡中皂苷合成关键酶基因表达及皂苷含量的影响[J]. 中国中药杂志, 2016,41(4):643-647.
[15]	Yang L, Wen K S, Ruan X , et al. Response of plant secondary metabolites to environmental factors[J]. Molecules, 2018,23(4):762. doi: 10.3390/molecules23040762 URL
[16]	中国科学院中国植物志编辑委员会. 中国植物志[M]. 北京: 科学出版社, 1978,15(2):92.
[17]	中国科学院西北植物研究所. 秦岭植物志[M]. 北京: 科学出版社, 1976,1(1):354.
[18]	宋小妹, 刘海静 . 太白七药研究与应用[M]. 北京: 人民卫生出版社, 2011: 132-139.
[19]	国家药典委员会. 中华人民共和国药典(一部)[M]. 北京: 中国医药科技出版社, 2015: 260.
[20]	Sevik H, Cetin M . Effects of water stress on seed germination for select landscape plants[J]. Polish Journal of Environmental Studies, 2015,24(2):689-693.
[21]	党云萍, 李春霞, 刘东雄 . 水分胁迫对植物生理生化研究进展[J]. 陕西农业科学, 2012,58(5):89-93.
[22]	刘倩, 左应梅, 杨维泽 , 等. 水分胁迫后复水对滇重楼生长以及皂苷积累的影响[J]. 中药材, 2018,41(10):2277-2281.
[23]	Wu X M, Zuo Z T, Zhang Q Z , et al. Effect of provenance and water stress on biomass and polyphyllin content in the medicinal plant Paris polyphylla Smith var. yunnanensis[J]. Acta Scientiarum Polonorum Hortorum Cultus, 2019,18(2):171-191.
[24]	李莉, 钟章成 . 诸葛菜对水分胁迫的生理生化反应和调节适应能力[J]. 西南师范大学学报:自然科学版, 2000,25(1):33-37.
[25]	王娟, 李德全 . 逆境条件下植物体内渗透调节物质的积累与活性氧代谢[J]. 植物学通报, 2001,18(4):459-465.

水分梯度	处理时间
水分梯度	2 h	6 h	12 h	24 h	3 d	5 d
CK	0.825a	0.663b	0.856b	0.897b	0.777b	0.635c
5% PEG	0.567c	0.515c	0.559c	1.657a	0.744b	0.547d
10% PEG	0.726b	0.867a	1.070a	0.457b	0.854b	0.950b
15% PEG	0.860a	0.613bc	0.458c	1.941a	1.366a	1.053a

水分梯度	处理时间
水分梯度	2 h	6 h	12 h	24 h	3 d	5 d
CK	0.825a	0.663b	0.856b	0.897b	0.777b	0.635c
5% PEG	0.567c	0.515c	0.559c	1.657a	0.744b	0.547d
10% PEG	0.726b	0.867a	1.070a	0.457b	0.854b	0.950b
15% PEG	0.860a	0.613bc	0.458c	1.941a	1.366a	1.053a

水分梯度	处理时间
水分梯度	2 h	6 h	12 h	24 h	3 d	5 d
CK	35.76b	28.91c	29.66c	41.04b	44.02b	47.15a
5% PEG	31.67c	27.21c	28.95c	37.34c	43.27b	40.31d
10% PEG	34.94b	33.66a	37.99b	48.01a	43.93b	44.84b
15% PEG	40.27a	30.75b	42.69a	40.81b	51.85a	43.68c

水分梯度	处理时间
水分梯度	2 h	6 h	12 h	24 h	3 d	5 d
CK	35.76b	28.91c	29.66c	41.04b	44.02b	47.15a
5% PEG	31.67c	27.21c	28.95c	37.34c	43.27b	40.31d
10% PEG	34.94b	33.66a	37.99b	48.01a	43.93b	44.84b
15% PEG	40.27a	30.75b	42.69a	40.81b	51.85a	43.68c

水分梯度	处理时间
水分梯度	2 h	6 h	12 h	24 h	3 d	5 d
CK	53.89c	38.43a	91.36b	62.45c	68.24b	55.85c
5% PEG	74.73b	42.31a	114.41a	70.09b	52.87d	34.15d
10% PEG	90.93a	31.92b	82.64b	66.68bc	60.00c	75.00b
15% PEG	91.19a	39.05a	111.14a	87.41a	110.41a	145.96a