Transcription Factor BvM14-Dof 3.4 in Response to Salt Stress: Functional Study

doi:10.11924/j.issn.1000-6850.casb2021-0262

Abstract

Abstract:

Dof family transcription factors are plant specific transcription factors, which play an important role in stress response, seed germination, nitrogen assimilation, photosynthesis and other biological processes. In order to explore the biological function of transcription factor BvM14-Dof3.4 in response to salt stress, the sugar beet M14 line with strong salt tolerance was used as the experimental material. The BvM14-Dof3.4 gene was heterologously expressed in wild type Arabidopsis thaliana by inflorescence impregnation. After 150 mmol/L NaCl treatment, it was found that the root growth of transgenic Arabidopsis thaliana under salt stress was promoted, the fresh and dry weight was increased by the heterologous expression of BvM14-Dof3.4 gene; the K⁺/Na⁺ ratio, the content of betaine and the enzyme activities of SOD and POD in heterologous expression plants were increased by 1.3 fold, 1.1 fold and 1.2 fold, respectively compared with the wild type Arabidopsis thaliana, which reduced the damage of heterologous expression Arabidopsis plants caused by salt stress. These results showed that BvM14-Dof3.4 gene was responsive to salt stress and the heterologous expression of BvM14-Dof3.4 gene could improve the salt tolerance of Arabidopsis thaliana. The study is of great significance for the mining of high quality gene resources of sugar beet M14 and the genetic improvement of stress resistance of cultivated sugar beet.

Key words: sugar beet M14 line, transcription factor, BvM14-Dof3.4 gene, salt stress, heterologous expression

CLC Number:

S566.3

Du Xiaoxue, Huang Yuanyuan, Ma Chunquan, Li Haiying. Transcription Factor BvM14-Dof 3.4 in Response to Salt Stress: Functional Study[J]. Chinese Agricultural Science Bulletin, 2021, 37(21): 119-125.

Figures/Tables 6

References 32

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引物功能	引物名称	序列
PCR引物	BvM14-Dof3.4-S	5'-ATGGGTGGAGGCGGAGGC-3'
PCR引物	BvM14-Dof3.4-AS	5'-TCACTTGAGACCATTGGCTGATG-3'
荧光定量PCR引物	18S-S	5'-CCCCAATGGATCCTCGTT A-3'
	18S-AS	5'-TGACGGAGAATTAGGGTT CG-3'
	dof -S	5'-GTGGAGGTGGTGGGTTTA-3'
	dof-AS	5'-TCCGTTTTCATTATTATT-3'
构建35S::pCAMBIA1300-BvM14-Dof3.4引物	1300-S	5'-CGAGCTCATGGGTGGAGGCGGAGGCGGAGGT-3'
构建35S::pCAMBIA1300-BvM14-Dof3.4引物	1300-AS	5'-CGAGATCTTCACTTGAGACCATTGGCTGATGT-3'

引物功能	引物名称	序列
PCR引物	BvM14-Dof3.4-S	5'-ATGGGTGGAGGCGGAGGC-3'
PCR引物	BvM14-Dof3.4-AS	5'-TCACTTGAGACCATTGGCTGATG-3'
荧光定量PCR引物	18S-S	5'-CCCCAATGGATCCTCGTT A-3'
	18S-AS	5'-TGACGGAGAATTAGGGTT CG-3'
	dof -S	5'-GTGGAGGTGGTGGGTTTA-3'
	dof-AS	5'-TCCGTTTTCATTATTATT-3'
构建35S::pCAMBIA1300-BvM14-Dof3.4引物	1300-S	5'-CGAGCTCATGGGTGGAGGCGGAGGCGGAGGT-3'
构建35S::pCAMBIA1300-BvM14-Dof3.4引物	1300-AS	5'-CGAGATCTTCACTTGAGACCATTGGCTGATGT-3'