Changes of Phytohormone and Structure in Xylem During Adventitious Root Production from the Stem of Parthenocissus tricuspidata Planch on the Rock Wall

doi:10.11924/j.issn.1000-6850.casb2021-0055

Abstract

Abstract:

To explore the spontaneously produced adventitious roots in the woody stems of Parthenocissus tricuspidata Planch on the rock wall, three kinds of stems were used as test materials. The hormone content in the xylem of the stem and the hormone level during the formation and development of adventitious root primordium induced by exogenous hormone were detected by enzyme-linked immunosorbent assay (Elisa). The results showed that the adventitious root primordium in the stem of annual Parthenocissus tricuspidata Planch belonged to internal origin. In the xylem of each test wood, the highest auxin (IAA) was 11.486 μg/g and the lowest was 6.255 μg/g counted on fresh weight. The highest of cytokinin (CTK) was 232.295 ng/g and the lowest was 150.235 ng/g counted on fresh weight, but the highest CTK was 232.295 ng/g when the adventitious root primordium was produced in the stem of 1-year-old Parthenocissus tricuspidata Planch treated with the exogenous hormone. The content of jasmonic acid (JA) was much lower than the 10-100 ng/g range of plant vegetative organs according to fresh weight, but the concentration in the rooting stem was much higher than that in the non-rooting stem. However, the content of methyl jasmonate (Me-JA) in the xylem of each test wood was very low, but the concentration in the rooting stem was much higher than that in the non-rooting stem. The IAA/CTK in the xylem of Rhizoma Parthenocissus tricuspidata Planch reached 27-76, and the concentration of hormone in the xylem of the rooting stem was much higher than that of the non-rooting stem, and the hormone concentration increased significantly when the stem produced adventitious root, which indicated that the spontaneous production of the stem adventitious root required the synergistic action of many kinds of endogenous hormones.

Key words: Parthenocissus tricuspidata Planch, woody growth stems, adventitious root primordia, endogenous hormones

CLC Number:

S718.43

ZHAO Shuang, WANG Huan, BU Xiaoying, DONG Aiwen. Changes of Phytohormone and Structure in Xylem During Adventitious Root Production from the Stem of Parthenocissus tricuspidata Planch on the Rock Wall[J]. Chinese Agricultural Science Bulletin, 2021, 37(36): 80-86.

Figures/Tables 11

References 28

[1]	邱靖. 三种垂直绿化植物净化大气中SO₂能力研究[D]. 南京:南京林业大学, 2009.
[2]	万欣. 三种垂直绿化植物叶片对重金属富集能力研究[D]. 南京:南京林业大学, 2009.
[3]	安凤生, 孙培福, 彭志成. 爬山虎属植物在园林中应用及生态效果[J]. 黑龙江科技信息, 2011(7):231.
[4]	韩庆典, 谢宝东. 3种绿化藤本植物降温增湿效应的研究[J]. 中国农学通报, 2014, 30(31):224-228.
[5]	张仪东, 王玲玲, 王杰, 等. 临沂市垂直绿化植物的现状及应用研究[J]. 安徽农业科学, 2018, 46(8):118-120.
[6]	肖永新, 邵君霞. 爬山虎在郑州的应用形式及栽植方式[J]. 现代园艺, 2016(7):132-133.
[7]	董爱文, 赵虹桥, 卜晓英, 等. 爬山虎属三种植物吸盘的解剖学研究[J]. 热带亚热带植物学报, 2009, 17(1):38-42.
[8]	蒋丽明, 张春英, 吕梁, 等. 丽水市城市园林垂直绿化植物的选择与应用研究[J]. 现代园艺, 2019(4):134-136.
[9]	李月娟, 王东雪, 魏育, 等. 不同外源激素处理下香花油茶扦插生根效果的综合评价[J]. 广西林业科学, 2020, 49(1):49-53.
[10]	郑鑫华, 董琼, 段华超, 等. 3种植物生长调节剂对树头菜扦插生根的影响[J]. 植物研究, 2020, 40(2):202-208.
[11]	秦爱丽, 简尊吉, 马凡强, 等. 母树年龄、生长调节剂、容器与基质对崖柏嫩枝扦插的影响[J]. 林业科学, 2018, 54(7):40-50.
[12]	任飞, 吴德军, 李庆华, 等. 黑松嫩枝扦插繁育技术研究[J]. 中国农学通报, 2021, 37(1):27-31.
[13]	李晗, 杨丹婷, 孟佳美, 等. LED不同光质对菘蓝愈伤组织诱导及再生的影响[J]. 中国农学通报, 2018, 34(36):65-69.
[14]	周鹏, 张敏, 吴双竹, 等. LED光质对乌饭树组培苗茎段增殖和生根的影响[J]. 植物研究, 2018, 38(5):697-703.
[15]	蔄胜军, 王志英, 苏晓华, 等. 杨树花枝盆栽加温促进生根人工杂交育种技术[J]. 林业科学研究, 2013, 26(1):101-106.
[16]	QUAN J, ZHANG C, ZHANG S, et al. Molecular cloning and expression analysis of the MTN gene during adventitious root development in IBA-induced tetraploid black locust[J]. Gene, 2014(553):140-150.
[17]	LAURA F, GIUSEPPINA F, CLAIRE K, et al. Adventitious rooting is enhanced by methyl jasmonate in tobacco thin cell layers[J]. Planta, 2009, 23:155-168.
[18]	ZHU X, CHAI S, CHEN L, et al. Induction and origin of adventitious roots from chimeras of Brassica juncea and Brassica oleracea[J]. Plant cell tiss organ cult, 2010, 10(1):287-294.
[19]	李丰, 张丽霞. 茶树空中压条不定根原基发育影响因子研究[J]. 山东农业科技, 2011(9):41-44.
[20]	张帅. 蛇皮果高空压条繁殖技术的研究[D]. 北京:中国林业科学研究院, 2013.
[21]	葛萌, 程平, 李宏, 等. 欧洲椴树分段压条繁殖技术的研究[J]. 中国农学通报, 2019, 35(15):104-109.
[22]	王欣, 李倩, 袁雪涛, 等. 五叶地锦高空压条生根条件优化[J]. 分子植物育种, 2020, 43(2):1-13.
[23]	冯大领, 李云, 孙振元. 爬山虎离体培养的初步研究[J]. 河北林果研究, 2005, 20(2):99-102,106.
[24]	相卫国, 李倩, 郝文芳, 等. 爬山虎扦插繁殖的研究[J]. 中国农学通报, 2006, 22(1):223-225.
[25]	曲建东, 高赟. 植物生长素与激动素对爬山虎嫩枝扦插生根的影响[J]. 农业科技与信息, 2012(17):55-56.
[26]	顾慧, 李金金, 张光泉, 等. “花叶”爬山虎的扦插繁殖[J]. 中国花卉园艺, 2018(8):45-47.
[27]	卜晓英, 董爱文, 管琼玉, 等. 荧光显微技术与HPLC联用法快速检测虎杖组织器官中白藜芦醇的分布与含量[J]. 中药材, 2012, 35(12):1909-1913.
[28]	QUAN J, ZHANG C, ZHANG S, et al. Molecular cloning and expression analysis of the MTN gene during adventitious root development in IBA-induced tetraploid black locust[J]. Gene, 2014(553):140-150.

样品号	IAA	CTK	JA	Me-JA
1	0.586±0.008 a A	0.719±0.007 a A	1.036±0.036 a A	0.471±0.007 a A
2	0.538±0.019 a	0.639±0.010 b	0.893 ±0.00 c C	0.374±0.003 b
3	0.431±0.003 c C	0.564±0.007 c	0.851±0.003 b	0.418±0.006 b
4	0.571±0.012 a	0.705±0.011 a	0.961±0.017 b B	0.438±0.00 a
5	0.573±0.005 a A	0.859±0.017 a A	0.990±0.037 a A	0.495±0.010 a A
6	0.484±0.015 a A	0.701±0.013 a A	0.812±0.008 a A	0.474±0.017 a
7	0.327±0.010 b B	0.604±0.003 b	0.716±0.020 b B	0.360±0.004 b
8	0.489±0.011 b B	0.691±0.011 b	0.959±0.011 a	0.326±0.003 c
9	0.450±0.004 a	0.602±0.011 b	0.964±0.015 a	0.437±0.005 a

样品号	IAA	CTK	JA	Me-JA
1	0.586±0.008 a A	0.719±0.007 a A	1.036±0.036 a A	0.471±0.007 a A
2	0.538±0.019 a	0.639±0.010 b	0.893 ±0.00 c C	0.374±0.003 b
3	0.431±0.003 c C	0.564±0.007 c	0.851±0.003 b	0.418±0.006 b
4	0.571±0.012 a	0.705±0.011 a	0.961±0.017 b B	0.438±0.00 a
5	0.573±0.005 a A	0.859±0.017 a A	0.990±0.037 a A	0.495±0.010 a A
6	0.484±0.015 a A	0.701±0.013 a A	0.812±0.008 a A	0.474±0.017 a
7	0.327±0.010 b B	0.604±0.003 b	0.716±0.020 b B	0.360±0.004 b
8	0.489±0.011 b B	0.691±0.011 b	0.959±0.011 a	0.326±0.003 c
9	0.450±0.004 a	0.602±0.011 b	0.964±0.015 a	0.437±0.005 a