Current Situation and Virus Identification of Sweet Potato Virus Disease (SPVD) in Shaanxi Province

doi:10.11924/j.issn.1000-6850.casb2021-1164

Abstract

Abstract:

To clarify the occurrence range and severity of sweet potato virus disease (SPVD) in Shaanxi Province, 98 samples of sweet potato suspected of virus disease were collected from seven cities in the province, including Xi’an, Xianyang, Weinan, Yulin, Shangluo, Ankang and Baoji, and tested for SPVD by using multiplex RT-PCR assay. The results showed that the total virus detection rate of the samples was 52.04%, and sweet potato chlorotic stunt virus (SPCSV) was detected in 38 samples (38.78%); sweet potato feathery mottle virus (SPFMV) was detected in 25 samples (25.52%). Among the 51 samples detected with viruses, 38 samples were infested with a single virus and 13 samples were infested with a combination of two viruses, and the detection rate of SPVD was 13.27%. Except that no SPFMV-CH2 strain was detected in Xianyang, SPCSV and SPFMV-CH and CH2 strains were all detected in other six cities. SPCSV was detected in 2 of the 46 breeding materials, with a detection rate of 4.35%, indicating that SPVD has occurred widely in Shaanxi Province, and the incidence is rather serious.

Key words: Shaanxi Province, sweet potato virus disease (SPVD), occurrence range, virus identification

CLC Number:

S432.4

YANG Yiwei, LIU Chen, WEI Peiyao, LI Yingmei. Current Situation and Virus Identification of Sweet Potato Virus Disease (SPVD) in Shaanxi Province[J]. Chinese Agricultural Science Bulletin, 2022, 38(33): 109-115.

Figures/Tables 11

References 20

[1]	GIBSON R W, MPEMBE I, ALICAI T, et al. Symptoms, aetiology and serological analysis of sweet potato virus disease in Uganda[J]. Plant pathology, 1998, 47(1):95-102. doi: 10.1046/j.1365-3059.1998.00196.x URL
[2]	HAHN S K. Effects of viruses (SPVD) on growth and yield of sweet potato[J]. Experimental agriculture, 1979, 15(3):253-256. doi: 10.1017/S0014479700010310 URL
[3]	GUTIERRES D L, FUENTES S, SAIAZAR L. Sweet potato virus disease(SPVD):distribution, incidence, and effect on sweet potato yield in Peru[J]. Plant disease, 2003, 87(3):297-302. doi: 10.1094/PDIS.2003.87.3.297 URL
[4]	NGEVE J M. Yield, stability and yield depression in sweet potato cultivars susceptible to the sweet potato virus disease[J]. Journal of horticultural science, 1990, 65(2):225-230. doi: 10.1080/00221589.1990.11516051 URL
[5]	张振臣, 乔奇, 秦艳红, 等. 中国发现由甘薯褪绿矮化病毒和甘薯羽状斑驳病毒协生共侵染引起的甘薯病毒病害[J]. 植物病理学报, 2012, 42(03):328-333.
[6]	姜珊珊, 谢礼, 吴斌, 等. 山东甘薯主要病毒的鉴定及多样性分析[J]. 植物保护学报, 2017, 44(1):93-102.
[7]	彭小琴, 王浩然, 张俊, 等. 湖北甘薯病毒病的检测与鉴定[J]. 中国植保导刊, 2017, 37(8):20-23.
[8]	黄利利, BINHDAN P, 何芳练, 等. 广西甘薯病毒病的病原病毒种类检测[J]. 基因组学与应用生物学, 2016, 35(5):1213-1218.
[9]	VALVERDE R A, SIM J, LOTRAKUL P. Whitefly transmissionof sweet potato viruse[J]. Virus research, 2004, 99(1):123-128.
[10]	TAIRO F, MUKASA S B, JONES R A, et al. Unravelling the genetic diversity of the three main viruses involved in Sweet Potato Virus Disease (SPVD), and its practical implications[J]. Molecular plant pathology, 2005, 6(2):199-211. doi: 10.1111/j.1364-3703.2005.00267.x URL
[11]	王晓华, 张振臣, 乔奇, 等. 甘薯羽状斑驳病毒外壳蛋白基因的分子变异[J]. 植物保护, 2012, 38(2):114-116,123.
[12]	KOKKINOS C D, CLARK C A. Interactions among sweet potato chlorotic stunt virus and different potyviruses and potyvirus strains infecting sweet potato in the United States[J]. Plant disease, 2006, 90:1347-1352. doi: 10.1094/PD-90-1347 URL
[13]	王钊, 高文川, 刘明慧, 等. 陕西省甘薯产业现状及发展对策[J]. 中国种业, 2017(6):25-27.
[14]	张盼, 兰新芝, 乔奇, 等. 甘薯病毒病害(SPVD)的多重RT-PCR检测方法及其应用[J]. 植物保护, 2013, 39(2):86-90,100.
[15]	王爽, 刘顺通, 乔奇, 等. 甘薯病毒病害SPVD抗性鉴定方法及产量损失估计[J]. 植物保护学报, 2014, 41(2):176-181.
[16]	唐启义, 冯明光. 实用统计分析及其DPS数据处理系统[M]. 北京: 科学出版社, 2002.
[17]	张新新, 王旭芳, 林坚淳, 等. 甘薯毁灭性病毒病害(SPVD)的研究进展[J]. 中国农学通报, 2019, 35(1):118-126.
[18]	李英梅, 白青, 王周平, 等. 烟粉虱与番茄黄化曲叶病毒病发生关系研究[J]. 中国农学通报, 2019, 35(4):102-107.
[19]	代惠洁. Q烟粉虱对番茄褪绿病毒的传播特性及其转录组分析[D]. 泰安: 山东农业大学, 2018.
[20]	李正跃, 张青文. 烟盲蝽田间种群动态及不同龄期与病毒病传播的关系[J]. 中国农业大学学报, 2005(2):26-29.

病毒	引物名称	引物序列	PCR片段大小/bp	Tm值
SPCSV	CSV1	5’-AGTGGTGAYGTAATAGTCGGTGG-3’	365	61.06
SPCSV	CSV2	5’-GCTAACGATTCACADACAGACTTCA-3’		59.24
SPFMV	FMV1	5’-GARCARTTYCRMGCATGGTATGA-3’	228	59.28
	FMV2	5’-GAAGTGYGTCATRATYTGCCTAA-3’		57.50
	FMVCH	5’-GCGTGATACGAGCAAAGAAAAGG-3’	457	57.80
	FMVCH2	5’-CCAAAAGGGGCATTTACAGCACC-3’	570	59.60

病毒	引物名称	引物序列	PCR片段大小/bp	Tm值
SPCSV	CSV1	5’-AGTGGTGAYGTAATAGTCGGTGG-3’	365	61.06
SPCSV	CSV2	5’-GCTAACGATTCACADACAGACTTCA-3’		59.24
SPFMV	FMV1	5’-GARCARTTYCRMGCATGGTATGA-3’	228	59.28
	FMV2	5’-GAAGTGYGTCATRATYTGCCTAA-3’		57.50
	FMVCH	5’-GCGTGATACGAGCAAAGAAAAGG-3’	457	57.80
	FMVCH2	5’-CCAAAAGGGGCATTTACAGCACC-3’	570	59.60

采样点		烟粉虱种群数量/（头/三叶）	蚜虫种群数量/（头/三叶）
西安市	临潼区	15.6±1.51fg	10.3±1.57cde
西安市	长安区	25.8±5.30de	9.8±2.31cde
咸阳市	杨凌区	16.4±4.08fg	10.6±2.51cde
咸阳市	兴平市	21.2±4.95efg	13.5±3.5bcde
延安市	宝塔区	17.7±2.56efg	6.3±4.88e
延安市	延川县	15.3±3.01fg	8.2±4.51de
渭南市	澄城县	23.4±3.67ef	15.3±4.68bcde
	合阳县	12.8±2.08g	13.3±2.86bcde
	大荔县	15.4±2.95fg	24.6±4.76a
商洛市	商州区	32.3±2.34cd	21.5±9.58ab
商洛市	洛南县	43.7±3.25b	19.8±3.54ab
安康市	石原县	35.6±4.08c	14.5±4.44bcde
安康市	紫阳县	44.6±3.50b	19.8±7.47ab
宝鸡市	岐山县	47.8±8.84b	17.8±4.17abc
宝鸡市	眉县	58.3±7.78a	16.3±3.75abcd

采样点		烟粉虱种群数量/（头/三叶）	蚜虫种群数量/（头/三叶）
西安市	临潼区	15.6±1.51fg	10.3±1.57cde
西安市	长安区	25.8±5.30de	9.8±2.31cde
咸阳市	杨凌区	16.4±4.08fg	10.6±2.51cde
咸阳市	兴平市	21.2±4.95efg	13.5±3.5bcde
延安市	宝塔区	17.7±2.56efg	6.3±4.88e
延安市	延川县	15.3±3.01fg	8.2±4.51de
渭南市	澄城县	23.4±3.67ef	15.3±4.68bcde
	合阳县	12.8±2.08g	13.3±2.86bcde
	大荔县	15.4±2.95fg	24.6±4.76a
商洛市	商州区	32.3±2.34cd	21.5±9.58ab
商洛市	洛南县	43.7±3.25b	19.8±3.54ab
安康市	石原县	35.6±4.08c	14.5±4.44bcde
安康市	紫阳县	44.6±3.50b	19.8±7.47ab
宝鸡市	岐山县	47.8±8.84b	17.8±4.17abc
宝鸡市	眉县	58.3±7.78a	16.3±3.75abcd

样品采集地		西安市	咸阳市	延安市	渭南市	商洛市	安康市	宝鸡市	总计
采集的样品数/个		13	10	10	14	16	18	17	98
检测到不同病毒的样品数及所占比例/ （个/%）	SPCSV	4/30.77	5/50.00	2/20.00	5/35.71	6/37.50	8/44.44	8/47.06	38/38.78
	SPFMV	3/23.08	2/20.00	2/20.00	4/28.57	6/37.50	3/16.67	5/29.41	25/25.51
	SPFMV-CH	2/15.38	2/20.00	1/10.00	3/21.43	1/6.25	1/5.55	5/29.41	17/17.35
	SPFMV-CH2	2/15.38	0/0.00	2/20.00	3/21.43	5/31.25	3/16.67	2/11.76	15/15.31
检出病毒样品数/个		5	6	4	7	9	9	11	51
样品检出率/%		38.46	60.00	40.00	50.00	56.25	50.00	64.71	52.04