PGPR in Atrazine Contaminated Soil: Effect on the Growth and Physiology of Zoysia japonica Steud

doi:10.11924/j.issn.1000-6850.casb2021-0272

Abstract

Abstract:

To provide a theoretical basis for planting Zoysia japonica Steud seedlings in atrazine contaminated soil, the effects of plant rhizosphere growth promoting bacteria (PGPR) on plant growth and physiological characteristics of Zoysia japonica Steud seedlings in atrazine contaminated soil were investigated. A two-factor experimental design was conducted. One-year-old Zoysia japonica Steud seedlings were used as materials, the seedlings were divided into two groups of PGPR (Pseudomonas fluorescens) inoculated and non-inoculated, and treated with atrazine (concentration gradient: 0, 3, 15, 75 mg/kg). The growth index, defensive enzyme activity, photosynthetic parameters and chlorophyll fluorescence of the seedlings under different atrazine concentrations were measured. The results showed that the inoculation of Pseudomonas fluorescens promoted the growth of Zoysia japonica Steud and alleviated the stress of atrazine at different concentrations. And the promoting effect of inoculation was the most obvious at the atrazine concentration of 3 mg/kg. Compared with the non-inoculated plants, the chlorophyll a and b of Zoysia japonica Steud leaves increased by 45.8% and 39.5%, respectively; net photosynthetic rate (Pn) increased by 184.83%; the maximum photochemical efficiency (Fv/Fm) and PSII potential activity (Fv/Fo) increased by 9.9% and 41.04%, respectively; peroxidase (POD) activity, soluble protein and proline content were 2.96, 1.94 and 2.37 times that of those of the control, The plant height, shoot fresh weight and dry weight of Zoysia japonica Steud increased by 71.43%, 47.69% and 78.13%, respectively. In conclusion, PGPR inoculation can promote the growth of Zoysia japonica Steud, increase chlorophyll content, improve photosynthesis, and has a significant effect on alleviating the toxicity of low concentration atrazine.

Key words: Pseudomonas fluorescen, atrazine, Zoysia japonica Steud, photosynthesis, physiology

CLC Number:

S688.4
X592

ZHENG Peifeng, JIANG Xiaolei, ZHAI Yanlin, GUO Shaoxia, LI Wei. PGPR in Atrazine Contaminated Soil: Effect on the Growth and Physiology of Zoysia japonica Steud[J]. Chinese Agricultural Science Bulletin, 2022, 38(5): 124-131.

Figures/Tables 7

References 28

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处理	莠去津/(mg/kg)	株高/cm	鲜重/g		干重/g
处理	莠去津/(mg/kg)	株高/cm	地上部	地下部	地上部	地下部
CK	0	21.4±1.2 d	13.03±1.25 b	14.27±0.97 ab	2.67±0.32 cd	1.83 ±0.09 b
	3	22.4±0.8 d	12.58±0.24 bc	12.52±0.98 bc	2.24±0.04 d	1.61± 0.04 bc
	15	21.7±0.6 d	9.61±0.85 c	11.70±0.55 c	2.01±0.17 d	1.42 ±0.05 cd
	75	17.8±1.1 e	5.78±0.88 d	7.45±0.40 e	2.57±0.73 cd	1.12 ±0.01 d
PGPR	0	37.5±0.7 a	19.92±0.36 a	15.29±0.84 a	4.66±0.16 a	2.34 ±0.20 a
	3	38.4±1.4 a	18.58±0.62 a	9.30±0.71 de	3.99±0.14 ab	1.15 ±0.11 d
	15	32.6±1.3 b	14.47±2.12 b	10.79±0.60 cd	3.49±0.08 bc	1.31 ±0.12 cd
	75	29.2±1.2 c	2.88±0.63 d	1.57±0.17 f	1.85±0.41 d	0.23± 0.01 e

处理	莠去津/(mg/kg)	株高/cm	鲜重/g		干重/g
处理	莠去津/(mg/kg)	株高/cm	地上部	地下部	地上部	地下部
CK	0	21.4±1.2 d	13.03±1.25 b	14.27±0.97 ab	2.67±0.32 cd	1.83 ±0.09 b
	3	22.4±0.8 d	12.58±0.24 bc	12.52±0.98 bc	2.24±0.04 d	1.61± 0.04 bc
	15	21.7±0.6 d	9.61±0.85 c	11.70±0.55 c	2.01±0.17 d	1.42 ±0.05 cd
	75	17.8±1.1 e	5.78±0.88 d	7.45±0.40 e	2.57±0.73 cd	1.12 ±0.01 d
PGPR	0	37.5±0.7 a	19.92±0.36 a	15.29±0.84 a	4.66±0.16 a	2.34 ±0.20 a
	3	38.4±1.4 a	18.58±0.62 a	9.30±0.71 de	3.99±0.14 ab	1.15 ±0.11 d
	15	32.6±1.3 b	14.47±2.12 b	10.79±0.60 cd	3.49±0.08 bc	1.31 ±0.12 cd
	75	29.2±1.2 c	2.88±0.63 d	1.57±0.17 f	1.85±0.41 d	0.23± 0.01 e

处理	莠去津/(mg/kg)	Chl a/(mg/kg)	Chl b/(mg/kg)	Chl (a+b)/(mg/kg)	Chl a/b	Car/(mg/kg)
CK	0	0.99±0.07 c	0.41±0.01 bc	1.40±0.10 c	2.46±0.183 ab	0.16±0.03 bc
	3	1.09±0.17 bc	0.43±0.05 bc	1.52±0.22 bc	2.53±0.131 ab	0.18±0.04 abc
	15	0.90±0.03 c	0.40±0.03 bc	1.30±0.01 c	2.25±0.232 b	0.15±0.03 bc
	75	0.91±0.07 c	0.39±0.01 bc	1.29±0.07 c	2.35±0.138 ab	0.19±0.02 c
PGPR	0	1.85±019 a	0.66±0.07 a	2.51±0.25 a	2.82±0.170 a	0.32±0.04 a
	3	1.59±0.11 ab	0.60±0.02 ab	2.20±0.13 ab	2.64±0.153 ab	0.22±0.07 ab
	15	1.64±0.19 a	0.74±0.01 a	2.37±0.28 a	2.23±0.083 b	0.24±0.04 ab
	75	0.56±0.01 c	0.36±0.02 c	0.92±0.03 c	1.56±0.059 c	0.05±0.01 c

处理	莠去津/(mg/kg)	Chl a/(mg/kg)	Chl b/(mg/kg)	Chl (a+b)/(mg/kg)	Chl a/b	Car/(mg/kg)
CK	0	0.99±0.07 c	0.41±0.01 bc	1.40±0.10 c	2.46±0.183 ab	0.16±0.03 bc
	3	1.09±0.17 bc	0.43±0.05 bc	1.52±0.22 bc	2.53±0.131 ab	0.18±0.04 abc
	15	0.90±0.03 c	0.40±0.03 bc	1.30±0.01 c	2.25±0.232 b	0.15±0.03 bc
	75	0.91±0.07 c	0.39±0.01 bc	1.29±0.07 c	2.35±0.138 ab	0.19±0.02 c
PGPR	0	1.85±019 a	0.66±0.07 a	2.51±0.25 a	2.82±0.170 a	0.32±0.04 a
	3	1.59±0.11 ab	0.60±0.02 ab	2.20±0.13 ab	2.64±0.153 ab	0.22±0.07 ab
	15	1.64±0.19 a	0.74±0.01 a	2.37±0.28 a	2.23±0.083 b	0.24±0.04 ab
	75	0.56±0.01 c	0.36±0.02 c	0.92±0.03 c	1.56±0.059 c	0.05±0.01 c

处理	莠去津/(mg/kg)	MDA/(μmol/g)		相对电导率/%
处理	莠去津/(mg/kg)	叶	根	叶	根
CK	0	7.20±2.17 ab	4.89±0.24 b	0.68±0.01 bc	0.59±0.04 cd
	3	10.11±0.34 ab	5.29±0.54 b	0.51±0.04 d	0.64±0.02 bcd
	15	14.67±2.10 a	5.67±0.21 ab	0.60±0.03 c	0.73±0.03 ab
	75	5.72±3.30 b	6.68±0.46 a	0.73±0.01 b	0.55±0.01 d
PGPR	0	6.56±2.40 ab	5.29±0.63 b	0.40±0.00 e	0.81±0.09 a
	3	7.34±2.59 ab	4.58±0.07 b	0.50±0.02 d	0.41±0.05 e
	15	7.48±2.62 ab	5.71±0.26 ab	0.70±0.02 b	0.71±0.01 abc
	75	10.09±1.04 ab	6.58±0.15 a	0.89±0.05 a	0.75±0.01 ab