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Chinese Agricultural Science Bulletin ›› 2023, Vol. 39 ›› Issue (14): 144-151.doi: 10.11924/j.issn.1000-6850.casb2022-0443

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Effects of Dietary Rhodiola rosea on Antioxidant System Function and Salinity Sudden Change Adaptation of Litopenaeus vannamei

ZHOU Wenchuan1(), WANG Yun2(), DUAN Yafei2, WANG Jun2, ZHOU Chuanpeng2, HUANG Zhong2   

  1. 1 Shenzhen Fisheries Development Center, Shenzhen, Guangdong 518067
    2 South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, Guangzhou 510300
  • Received:2022-06-06 Revised:2022-10-28 Online:2023-05-09 Published:2023-05-09

Abstract:

To investigate the effect of Rhodiola rosea extract on antioxidant system function and salinity change adaptation of Litopenaeus vannamei, four diets were prepared with R. rosea inclusion at 0 (control diet), 0.3, 1 and 3 g/kg and fed to shrimp for 28 days. Then low salinity (10) change adaptation test was conducted and antioxidant system function including total antioxidant status (T-AOC), glutathione peroxidase (GSH-Px), catalase (CAT) in serum as well as gene expression of GSH-Px and CAT in hemocyte of shrimp were analyzed. Two-way ANOVA analysis results showed that R. rosea extract levels and times (rearing and salinity change time) both played a significant role on the effect of GSH-Px and CAT activities in serum and gene expression levels of GSH-Px and CAT in hemocyte of shrimp (P<0.001). There was a significant interaction between the effects of two factors (R. rosea extract level & rearing and salinity sudden change times) on the activities of T-AOC, GSH-Px, CAT and gene expression levels of GSH-Px and CAT. Simple effect analysis indicated that the effect of rearing and salinity change times on T-AOC activity in serum of shrimp was the lowest when 0.3 g/kg R. rosea extract was added into feed (F=2.338,P<0.001). Moreover, the effects of rearing and salinity change times on the activities of GSH-Px (F=5696.236, P<0.001), CAT (F=0.314,P<0.001) in serum and gene expression levels of GSH-Px and CAT in hemocytes were relatively low. Diets containing 0.3 g/kg R. rosea extract are more suitable for maintaining the stability in the antioxidant defense system of L. vannamei and its adaptability to salinity sudden change.

Key words: Rhodiola rosea, Litopenaeus vannamei, antioxidant function, salinity sudden change