Effects of Dietary Rhodiola rosea on Antioxidant System Function and Salinity Sudden Change Adaptation of Litopenaeus vannamei

doi:10.11924/j.issn.1000-6850.casb2022-0443

Abstract

Abstract:

To investigate the effect of Rhodiola rosea extract on antioxidant system function and salinity change adaptation of Litopenaeus vannamei, four diets were prepared with R. rosea inclusion at 0 (control diet), 0.3, 1 and 3 g/kg and fed to shrimp for 28 days. Then low salinity (10) change adaptation test was conducted and antioxidant system function including total antioxidant status (T-AOC), glutathione peroxidase (GSH-Px), catalase (CAT) in serum as well as gene expression of GSH-Px and CAT in hemocyte of shrimp were analyzed. Two-way ANOVA analysis results showed that R. rosea extract levels and times (rearing and salinity change time) both played a significant role on the effect of GSH-Px and CAT activities in serum and gene expression levels of GSH-Px and CAT in hemocyte of shrimp (P<0.001). There was a significant interaction between the effects of two factors (R. rosea extract level & rearing and salinity sudden change times) on the activities of T-AOC, GSH-Px, CAT and gene expression levels of GSH-Px and CAT. Simple effect analysis indicated that the effect of rearing and salinity change times on T-AOC activity in serum of shrimp was the lowest when 0.3 g/kg R. rosea extract was added into feed (F=2.338，P<0.001). Moreover, the effects of rearing and salinity change times on the activities of GSH-Px (F=5696.236, P<0.001), CAT (F=0.314，P<0.001) in serum and gene expression levels of GSH-Px and CAT in hemocytes were relatively low. Diets containing 0.3 g/kg R. rosea extract are more suitable for maintaining the stability in the antioxidant defense system of L. vannamei and its adaptability to salinity sudden change.

Key words: Rhodiola rosea, Litopenaeus vannamei, antioxidant function, salinity sudden change

ZHOU Wenchuan, WANG Yun, DUAN Yafei, WANG Jun, ZHOU Chuanpeng, HUANG Zhong. Effects of Dietary Rhodiola rosea on Antioxidant System Function and Salinity Sudden Change Adaptation of Litopenaeus vannamei[J]. Chinese Agricultural Science Bulletin, 2023, 39(14): 144-151.

Figures/Tables 9

References 29

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原料及营养组成	对照	0.3 g/kg红景天	1 g/kg红景天	3 g/kg红景天
鱼粉	25	25	25	25
大豆粕	18	18	18	18
花生麸	16.4	16.4	16.4	16.4
面粉	23	22.97	22.90	22.70
啤酒酵母	5	5	5	5
虾头粉	5	5	5	5
大豆磷脂	1	1	1	1
鱼油	1	1	1	1
豆油	1	1	1	1
氯化胆碱	0.5	0.5	0.5	0.5
磷酸二氢钙	1	1	1	1
抗坏血酸酯	0.1	0.1	0.1	0.1
多维预混料^*	1	1	1	1
微量矿物元素预混料^**	1	1	1	1
红景天提取物	0	0.03	0.10	0.30
海藻酸钠	1	1	1	1
总量	100	100	100	100
粗蛋白	39.74	39.43	39.74	39.80
粗脂肪	7.31	7.24	7.26	7.30
粗灰分	11.20	11.34	11.33	11.02
水分	9.65	9.87	9.82	9.67

原料及营养组成	对照	0.3 g/kg红景天	1 g/kg红景天	3 g/kg红景天
鱼粉	25	25	25	25
大豆粕	18	18	18	18
花生麸	16.4	16.4	16.4	16.4
面粉	23	22.97	22.90	22.70
啤酒酵母	5	5	5	5
虾头粉	5	5	5	5
大豆磷脂	1	1	1	1
鱼油	1	1	1	1
豆油	1	1	1	1
氯化胆碱	0.5	0.5	0.5	0.5
磷酸二氢钙	1	1	1	1
抗坏血酸酯	0.1	0.1	0.1	0.1
多维预混料^*	1	1	1	1
微量矿物元素预混料^**	1	1	1	1
红景天提取物	0	0.03	0.10	0.30
海藻酸钠	1	1	1	1
总量	100	100	100	100
粗蛋白	39.74	39.43	39.74	39.80
粗脂肪	7.31	7.24	7.26	7.30
粗灰分	11.20	11.34	11.33	11.02
水分	9.65	9.87	9.82	9.67

基因名称	正向引物(5′-3′)	反向引物(5′-3′)	产物大小/bp	GenBank No.
CAT	TCAGCGTTTGGTGGAGAA	GCCTGGCTCATCTTTATC	147	AY973252
GPx	AGGGACTTCCACCAGATG	CAACAACTCCCCTTCGGTA	117	AY518322
β-actin	GCCCATCTACGAGGGATA	GGTGGTCGTGAAGGTGTAA	121	AF300705

基因名称	正向引物(5′-3′)	反向引物(5′-3′)	产物大小/bp	GenBank No.
CAT	TCAGCGTTTGGTGGAGAA	GCCTGGCTCATCTTTATC	147	AY973252
GPx	AGGGACTTCCACCAGATG	CAACAACTCCCCTTCGGTA	117	AY518322
β-actin	GCCCATCTACGAGGGATA	GGTGGTCGTGAAGGTGTAA	121	AF300705

分组		0 g/kg	0.3 g/kg	1 g/kg	3 g/kg	分组	F	P
养殖时间/d	0	11.81±0.44	11.39±0.20	11.49±0.19	11.64±0.01	红景天添加量	2.106	0.108
	7	12.20±0.05	12.76±0.13	12.40±0.30	12.40±0.55	养殖与盐度骤变时间	24.218	<0.001
	14	10.53±0.24	10.41±0.23	9.24±0.11	8.86±0.14	交互作用	3.621	<0.001
	21	9.55±0.27	10.68±0.71	9.77±0.51	11.53±0.72	-	-	-
	28	10.83±0.85	10.51±0.19	9.79±0.19	10.92±0.82	-	-	-
盐度骤变时间/h	24	13.41±0.04	10.76±0.09	12.98±0.53	11.51±0.65	-	-	-
	48	12.04±0.13	10.10±0.45	9.71±0.23	10.71±0.06	-	-	-
	72	9.22±0.20	10.07±0.76	10.24±0.23	10.14±0.37	-	-	-