Exopolysaccharides from Lactiplantibacillus plantarum HDL-03: Synthesis Conditions Optimization

doi:10.11924/j.issn.1000-6850.casb2023-0325

Abstract

Abstract:

In order to improve the yield of exopolysaccharide (EPS) of lactic acid bacteria and promote its development and application, Lactiplantibacillus plantarum HDL-03 was used as the test strain and the fermentation conditions of the EPS were optimized by single factor experiment to improve production. The results indicated that the optimal fermentation conditions of L. plantarum HDL-03 were sucrose 70 g/L, peptone 6 g/L, beef paste 8 g/L, yeast extract 7 g/L, sodium acetate 1 g/L, magnesium sulfate 0.3 g/L, dipotassium hydrogen phosphate 1 g/L, ammonium citrate 3 g/L and initial pH 6.5. Culture temperature was 30℃, shaking table rotation speed was 120 r/min and inoculum size was 3%. The final EPS yield of L. plantarum HDL-03 after optimization was 80.0 g/L, which was 2.3 times higher than the EPS yield before optimization. In this study, the yield of EPS produced by lactic acid bacteria was increased, which contributed to its separation, purification and industrial production.

Key words: Lactiplantibacillus plantarum, exopolysaccharide, single factor, fermentation conditions, optimization

ZHOU Bosen, CAO Huiying, QI Xintong, YU Liansheng, YANG Yi, GE Jingping, SONG Gang, DU Renpeng. Exopolysaccharides from Lactiplantibacillus plantarum HDL-03: Synthesis Conditions Optimization[J]. Chinese Agricultural Science Bulletin, 2024, 40(11): 14-21.

Figures/Tables 6

References 32

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