Bacillus velezensis YFB3-1: Optimization of Liquid Fermentation Conditions for High Density

doi:10.11924/j.issn.1000-6850.casb2022-0448

Abstract

Abstract:

The aim is to obtain high density Bacillus velezensis YFB3-1 biocontrol agent which can be used to prevent soil-borne diseases of crops. In this study, single factor and orthogonal experiments were used to optimize the liquid medium and culture conditions of strain YFB3-1. The results showed that strain YFB3-1 was cultured in liquid medium with yeast powder 8 g/L, compound amino acid 10 g/L, sodium chloride 1 g/L and magnesium sulfate (MgSO₄·7H₂O) 1 g/L, and when the fermentation conditions were initial pH of 7.0, inoculation amount of 2.5%, fermentation temperature of 30℃, rotation speed of 180 r/min and culture time of 48 h, the bacterial concentration was 2.89×10¹⁰ cfu/mL, and the optimal culture medium and culture conditions were obtained for the liquid fermentation of strain YFB3-1. Compared with beef extract peptone liquid medium (NB), in which the bacterial concentration was 4.77×10⁹ cfu/mL, the bacterial concentration under the fermentation conditions of this experiment increased by 5.06 times, and the fermentation efficiency could be improved in the same time. The study provides a theoretical basis and technical parameters for the industrial expansion and application of B. velezensis YFB3-1.

Key words: Bacillus velezensis, orthogonal test, high-density liquid fermentation, optimization of fermentation condition

LU Yaoxiong, LI Wei, ZHOU Hu, QU Yuguo, XIONG Youming, WANG Yunsheng. Bacillus velezensis YFB3-1: Optimization of Liquid Fermentation Conditions for High Density[J]. Chinese Agricultural Science Bulletin, 2023, 39(18): 123-130.

Figures/Tables 10

References 32

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水平	牛肉膏(A)	复合氨基酸(B)	氯化钠(C)	硫酸镁(D)
1	6	7.5	1	0.5
2	8	10	3	1
3	10	12	5	1.5

水平	牛肉膏(A)	复合氨基酸(B)	氯化钠(C)	硫酸镁(D)
1	6	7.5	1	0.5
2	8	10	3	1
3	10	12	5	1.5

编号	A	B	C	D	菌体浓度(×10⁹cfu/mL)
1	1	1	1	1	20.20±0.97d
2	1	2	2	2	25.32±0.84b
3	1	3	3	3	25.00±0.81b
4	2	1	2	3	23.08±0.87c
5	2	2	3	1	28.29±0.65a
6	2	3	1	2	28.44±0.52a
7	3	1	3	2	23.12±0.88c
8	3	2	1	3	28.32±0.63a
9	3	3	2	1	28.17±0.77a
K₁	23.51	22.14	25.65	25.56
K₂	26.60	27.31	25.52	25.63
K₃	26.54	27.20	25.47	25.47
R	3.10	5.17	0.18	0.16

编号	A	B	C	D	菌体浓度(×10⁹cfu/mL)
1	1	1	1	1	20.20±0.97d
2	1	2	2	2	25.32±0.84b
3	1	3	3	3	25.00±0.81b
4	2	1	2	3	23.08±0.87c
5	2	2	3	1	28.29±0.65a
6	2	3	1	2	28.44±0.52a
7	3	1	3	2	23.12±0.88c
8	3	2	1	3	28.32±0.63a
9	3	3	2	1	28.17±0.77a
K₁	23.51	22.14	25.65	25.56
K₂	26.60	27.31	25.52	25.63
K₃	26.54	27.20	25.47	25.47
R	3.10	5.17	0.18	0.16

主体间效应的检验
因变量:菌体浓度
来源	III型平方和	df	均方	F	Sig.
校正模型	313.258^a	8	39.157	66.589	.000
截距	24034.329	1	24034.329	40871.563	.000
A	78.685	2	39.342	66.903	.000
B	219.636	2	109.818	186.751	.000
C	.221	2	.111	.188	.830
D	.156	2	.078	.132	.877
误差	16.465	28	.588
总计	24210.253	37
校正的总计	329.723	36