Effects of Sulfur Level on Key Enzymes Activities in Carbon and Nitrogen Metabolism in Soybean Leaves

doi:10.11924/j.issn.1000-6850.casb2022-0720

Abstract

Abstract:

In order to explore the effects of different sulfur levels on the key enzyme activities in carbon and nitrogen metabolism in soybean leaves at seedling and flowering stages, soybean varieties ‘Laidou 2’ and ‘Hefeng 55’ were used as test materials. The data of key enzyme activities of carbon metabolism in soybean leaves at seedling and flowering stages were measured by watering nutrient solution. The GOGAT and SS enzyme activities of ‘Laidou 2’ and ‘Hefeng 55’ reached the maximum under 160 mg/L treatment. The peak value of GS enzyme activity of ‘Laidou 2’ was the strongest under 160 mg/L treatment, the peak value of ‘Hefeng 55’ in seedling stage was the highest under 260 mg/L treatment, and the peak value in flowering stage was the lowest under 20 mg/L treatment of sulfur nutrition. The SPS enzyme activity of ‘Laidou 2’ reached the maximum value under 40 mg/L treatment, while the peak value of ‘Hefeng 55’ at seedling stage was under 80 mg/L treatment with higher sulfur nutrition, and the peak value at flowering stage was under 260 mg/L treatment with the highest sulfur level. The results showed that the activities of glutamate synthase (GOGAT), glutamine synthetase (GS), sucrose phosphate synthase (SPS) and sucrose synthase (SS) at flowering stage were significantly higher than those at seedling stage, and the same rule was found among varieties.

Key words: sulphur levels, soybean, carbon and nitrogen metabolism, enzyme activity, seedling stage, flowering period

WANG Xiaojing, LI Yongping, SONG Shuang, ZHOU Qi, WANG Xin, DONG Shoukun. Effects of Sulfur Level on Key Enzymes Activities in Carbon and Nitrogen Metabolism in Soybean Leaves[J]. Chinese Agricultural Science Bulletin, 2024, 40(12): 113-118.

Figures/Tables 10

References 31

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序号	S/(mg/L)	MgSO₄/(mg/L)	MgCl₂/(mg/L)
1	20.0	75.231	714.500
2	40.0	150.462	654.569
3	80.0	300.925	535.556
4	160.0	601.850	297.531
5	260.0	978.006	0.0

序号	S/(mg/L)	MgSO₄/(mg/L)	MgCl₂/(mg/L)
1	20.0	75.231	714.500
2	40.0	150.462	654.569
3	80.0	300.925	535.556
4	160.0	601.850	297.531
5	260.0	978.006	0.0

无机盐	浓度/(mg/L)	无机盐	浓度/(mg/L)
KH₂PO₄	136.00	Na₂MoO₄·H₂O	0.03
CaCl₂	220.00	MnCl₂·H₂O	4.90
CuCl₂	0.05	H₃BO₃	2.86
NH₄NO₃	142.86	Fe-EDTA	-
ZnCl₂	0.10	-	-

无机盐	浓度/(mg/L)	无机盐	浓度/(mg/L)
KH₂PO₄	136.00	Na₂MoO₄·H₂O	0.03
CaCl₂	220.00	MnCl₂·H₂O	4.90
CuCl₂	0.05	H₃BO₃	2.86
NH₄NO₃	142.86	Fe-EDTA	-
ZnCl₂	0.10	-	-