Effect of Exogenous Melatonin on Germination and Resistance Ability of Peanut Seeds Under Drought Stress

doi:10.11924/j.issn.1000-6850.casb2023-0613

Abstract

Abstract:

The study aimed to explore the effect of exogenous melatonin on seed germination and resistance of peanut under drought stress. In this study, the drought-sensitive peanut variety ‘Jihua 5’ was used as the test material, and four treatments were set up, including blank water-soaked seed (CK), melatonin-soaked seed (T₁), 10% PEG-6000 treatment (T₂), and melatonin-soaked seed plus 10% PEG-6000 treatment (T₃), and four other treatments were used to determine the antioxidant physiological and biochemical indices after seed germination. The results showed that the 10% PEG-6000 was effective in simulating the drought environment. Meanwhile, melatonin concentration of 100 µmol/L was the best choice to promote peanut seed germination. Further analyzing the length and thickness of radicle and antioxidant physiological and biochemical indexes of peanut seeds under each treatment, we found that the application of 100 µmol/L melatonin under drought stress significantly enhanced the growth potential of radicle and increased the contents of SOD, POD and Pro, and effectively reduced the contents of MDA and H₂O₂ in peanut seeds. This study indicated that exogenous melatonin could effectively improve the germination of peanut seeds under drought stress, and provided a theoretical basis for further investigation of the regulatory mechanism of melatonin in promoting peanut seed germination under drought stress.

Key words: peanut seeds, melatonin, seed germination, drought stress, melatonin, resistance, antioxidant physiological and biochemical indicators

SUN Sainan, MIAO Penghui, QUAN Yuewei, MU Guojun, GUO Liguo, YANG Xinlei. Effect of Exogenous Melatonin on Germination and Resistance Ability of Peanut Seeds Under Drought Stress[J]. Chinese Agricultural Science Bulletin, 2024, 40(18): 38-44.

Figures/Tables 4

References 38

[1]	万书波, 张佳蕾. 中国花生产业降本增效新途径探讨[J]. 中国油料作物学报, 2019, 41(5):657-662. doi: 10.19802/j.issn.1007-9084.2019130
[2]	TANG J, BUSSO C A, JIANG D, et al. Seed burial depth and soil water content affect seedling emergence and growth of Ulmus pumila var. sabulosa in the horqin dandy land[J]. Sustainability, 2016, 8(1):68.
[3]	DOLFERUS R, THAVAMANIKUMAR S, SANGMA H, et al. Determining the genetic architecture of reproductive stage drought tolerance in wheat using a correlated trait and correlated marker effect model[J]. G3-genes genomes genetics, 2019, 9(2):473-489. doi: 10.1534/g3.118.200835 pmid: 30541928
[4]	范小玉, 陈雷, 刘卫星, 等. 氯化钙浸种对干旱胁迫下花生种子萌发及幼苗生理特性的影响[J]. 江苏农业科学, 2022, 50(8):101-105.
[5]	TAN D X, HARDELAND R, MANCHESTER L C, et al. Functional roles of melatonin in plants, and perspectives in nutritional and agricultural science[J]. Journal of experimental botany, 2011, 63(2):577-597.
[6]	LERNER A B, CASE J D, TAKAHASHI Y, et al. Isolation of melatonin, the pineal gland factor that lightens melanocytes[J]. Journal of the American chemical society, 1958, 80(10):2587.
[7]	WEI J, LI D X, ZHANG J R, et al. Phytomelatonin receptor PMTR1-mediated signaling regulates stomatal closure in Arabidopsis thaliana[J]. Journal of pineal research, 2018, 65(2):e12500.
[8]	IMRAN M, LATIF K A, SHAHZAD R, et al. Exogenous melatonin induces drought stress tolerance by promoting plant growth and antioxidant defence system of soybean plants[J]. Aob plants, 2021, 13(4):plab026.
[9]	MYRCH S J, SAXENA P K. Melatonin: a potential regulator of plant growth and development[J]. In vitro cellular & developmental biology-plant, 2002, 38(6):531-536.
[10]	POSMYK M M, BALABUSTA M, WIECZOREK M, et al. Melatonin applied to cucumber (Cucumis sativus L.) seeds improves germination during chilling stress[J]. Journal of pineal research, 2009, 46(2):214-223.
[11]	SIMLAT M, PTAK A, SKRZYPEK E, et al. Melatonin significantly influences seed germination and seedling growth of Stevia rebaudiana Bertoni[J]. Peer j, 2018,6:e5009.
[12]	ARNAO M B, HERNÁNDEZ-RUIZ J. Protective effect of melatonin against chlorophyll degradation during the senescence of barley leaves[J]. Journal of pineal research, 2009, 46(1):58-63. doi: 10.1111/j.1600-079X.2008.00625.x pmid: 18691358
[13]	SHI H T, RIEITER R J, TAN D X, et al. INDOLE-3-ACETIC ACID INDUCIBLE 17 positively modulates natural leaf senescence through melatonin -mediated pathway in Arabidopsis[J]. Journal of pineal research, 2015, 58(1):26-33.
[14]	ZHAO D, WANG H, CHEN S, et al. Phytomelatonin: an emerging regulator of plant biotic stress resistance[J]. Trends plant sci, 2021, 26(1):70-82. doi: 10.1016/j.tplants.2020.08.009 pmid: 32896490
[15]	BAI Y, XIAO S, ZHANG Z, et al. Melatonin improves the germination rate of cotton seeds under drought stress by opening pores in the seed coat[J]. Peer j, 2020,8:e9450.
[16]	马旭辉, 陈茹梅, 柳小庆, 等. 褪黑素对玉米幼苗根系发育和抗旱性的影响[J]. 生物技术通报, 2021, 37(2):1-14. doi: 10.13560/j.cnki.biotech.bull.1985.2020-0575
[17]	SADAK M S, ABDALLA A M, ELHAMID E M, et al. Role of melatonin in improving growth, yield quantity and quality of Moringa oleifera L. plant under drought stress[J]. Bulletin of the national research centre, 2020,44:18.
[18]	JAFARI M, SHAHSAVAR A. The effect of foliar application of melatonin on changes in secondary metabolite contents in two citrus species under drought stress conditions[J]. Frontiers in plant science, 2021,12:1509.
[19]	ZHANG N, ZHAO B, ZHANG H J, et al. Melatonin promotes water-stress tolerance, lateral root formation, and seed germination in cucumber (Cucumis sativus L.)[J]. Journal of pineal research, 2013,54:15-23.
[20]	LIU J, WANG W, WANG L, et al. Exogenous melatonin improves seedling health index and drought tolerance in tomato[J]. Plant growth regul, 2015,77:317-326.
[21]	LIU J, HASANUZZAMAN M, WEN H, et al. High temperature and drought stress cause abscisic acid and reactive oxygen species accumulation and suppress seed germination growth in rice[J]. Protoplasma, 2019, 256(5):1217-1227. doi: 10.1007/s00709-019-01354-6 pmid: 31001689
[22]	LUO Z, LIU H, LI W P, et al. Effects of reduced nitrogen rate on cotton yield and nitrogen use efficiency as mediated by application mode or plant density[J]. Field crops research, 2018,218:150-157.
[23]	ARNAO M B, HERNÁNDEZ-RUIZ J. Melatonin against environmental plant stressors: a review[J]. Current protein & peptide science, 2021, 22(5):413-429.
[24]	吕严. 褪黑素影响种子萌发的机制研究[D]. 昆明: 云南大学, 2019.
[25]	LI J, ZHAO C, ZHANG M, et al. Exogenous melatonin improves seed germination in Limonium bicolor under salt stress[J]. Plant signaling & behavior, 2019, 14(11):1659705.
[26]	李平, 谢淑芹, 方璐斌, 等. 干旱胁迫下不同褪黑素浓度处理下谷子种子萌发特性[J]. 山西农业大学学报(自然科学版), 2022, 42(5):76-83.
[27]	GUO Y, LI D, LIU L, et al. Seed priming with melatonin promotes seed germination and seedling growth of Triticale hexaploide L. under PEG-6000 induced drought stress[J]. Frontiers in plant science, 2022, 6(30):932912.
[28]	包宇, 罗庆熙, 黄娟, 等. 外源褪黑素对低温胁迫下番茄幼苗生理指标的影响[J]. 西南师范大学学报(自然科学版), 2013, 38(10):57-61.
[29]	HU Z, FAN J, XIE Y, et al. Comparative photosynthetic and metabolic analyses reveal mechanism of improved cold stress tolerance in bermudagrass by exogenous melatonin[J]. Plant physiol biochem, 2016,100:94-104.
[30]	YANG P M, HUANG Q C, QIN G Y, et al. Different drought-stress responses in photosynthesis and reactive oxygen metabolism between autotetraploid and diploid rice[J]. Photosynthetica, 2014, 52:193-202.
[31]	郝敬虹, 易旸, 尚庆茂, 等. 水杨酸处理对干旱胁迫下黄瓜幼苗氮素同化及其关键酶活性的影响[J]. 园艺学报, 2012, 39(1):81-90.
[32]	SU X, FAN X, SHAO R, et al. Physiological and iTRAQ-based proteomic analyses reveal that melatonin alleviates oxidative damage in maize leaves exposed to drought stress[J]. Plant physiol biochem, 2019,142:263-274.
[33]	ZHANG Z, LIU L, LI H, et al. Exogenous melatonin promotes the salt tolerance by removing active oxygen and maintaining ion balance in wheat (Triticum aestivum L.)[J]. Frontiers in plant science, 2022,12:787062.
[34]	TAN D X, MANCHESTER L C, REITER R J, et al. Melatonin directly scavenges hydrogen peroxide: a potentially new metabolic pathway of melatonin biotransformation[J]. Free radical biology and medicine, 2000, 29(11):1177-1185. doi: 10.1016/s0891-5849(00)00435-4 pmid: 11121726
[35]	王慧, 王冬梅, 张泽洲, 等. 外源褪黑素对干旱胁迫下黑麦草和苜蓿抗氧化能力及养分吸收的影响[J]. 应用生态学报, 2022, 33(5):1311-1319. doi: 10.13287/j.1001-9332.202205.004
[36]	HASAN K H, AHAMMED G J, SUN S C, et al. Melatonin inhibits cadmium translocation and enhances plant tolerance by regulating sulfur uptake and assimilation in Solanum lycopersicum L.[J]. Journal of agricultural and food chemistry, 2019,67:10563-10576.
[37]	HAYAT S, HAYAT Q, AIYEMENI M N, et al. Role of proline under changing environments: a review[J]. Plant signaling & behavior, 2012,7:1456-1466.
[38]	ULHASSAN Z, HUANG Q, GILL R A, et al. Protective mechanisms of melatonin against selenium toxicity in Brassica napus: insights into physiological traits, thiol biosynthesis and antioxidant machinery[J]. Bmc plant biology, 2019,19:507.