Effect of 137Cs-γ Irradiation on Seed Germination and Scion Growth in Macadamia Nut

doi:10.11924/j.issn.1000-6850.casb2023-0666

Abstract

Abstract:

In order to investigate the mutagenic effect of ¹³⁷Cs-γirradiation on seeds and scions of macadamia nut. In this study, the seeds and scions form self-bred macadamia variety‘Guire No.1’were treated with 0-250 Gy and 0-200 Gy radiation doses, to analyze the radiation effects on the seed germination, seedling growth and scion survival rate, respectively. The results showed that: (1)¹³⁷Cs-γhad a very significant inhibitory effect on germination and seedling formation of macadamia nut seeds within a certain dose range. The linear regression equation of radiation dose and germination rate was y=-2.5849x+231.14. The calculated semi-lethal dose and critical dose was 101.895 Gy and 127.744 Gy, respectively. (2) The effect of 150 Gy radiation on the leaf margin spines was significant, and there were fewer leaf margin spines than those of control. With a high dose of ¹³⁷Cs-γradiation, the phenotypes of treated seedlings leaves became shorter and wider, the plant height became shorter, and the stems became thinner compared with control. (3) Radiation reduced the survival rate of macadamia nut scions, and doses of 100 Gy radiation treatment reduced the survival rate of seedlings to 0. Macadamia nut scions growth well under 10-50 Gy treatment. The results showed that the appropriate dose range of macadamia nut seed and scion were 101.895-127.744 Gy and 10-50 Gy during ¹³⁷Cs-γradiation mutagenic breeding, respectively. This study provides a certain reference for the application of ¹³⁷Cs-γ irradiation in the macadamia nut breeding program.

Key words: ¹³⁷Cs-γ irradiation, macadamia nuts, seed germination, seedling growth, scion growth

TAN Qiujin, ZHOU Chunheng, HUAN Xiuju, XU Peng, ZHONG Jianzhang, HE Xianyang, HUANG Xiyun, YANG Xiaozhou, LIANG Ying. Effect of ¹³⁷Cs-γ Irradiation on Seed Germination and Scion Growth in Macadamia Nut[J]. Chinese Agricultural Science Bulletin, 2024, 40(23): 39-44.

Figures/Tables 6

References 29

[1]	TRUEMAN S J. The reproductive biology of macadamia[J]. Scientia horticulturae, 2013, 150:354-359.
[2]	谭秋锦, 王文林, 韦媛荣, 等. 澳洲坚果种质果实产量相关性状的多样性分析[J]. 果树学报, 2019, 36(12):1630-1637.
[3]	王文林, 张涛, 汤秀华, 等. 中国澳洲坚果产业概况与发展模式探索[J]. 农业研究与应用, 2022, 35(4):44-50.
[4]	谭秋锦, 韦媛荣, 潘贞珍, 等. 澳洲坚果种质果实数量性状分析与综合评价[J]. 植物遗传资源学报, 2023, 24(6):1615-1625.
[5]	柳觐, 倪书邦, 孔广红, 等. (60)Co-γ射线对不同品种澳洲坚果萌动种子的诱变效应[J]. 西南农业学报, 2019, 32(5):1149-1154.
[6]	OUSSAMA B, MINA A, LINDEN V M G, et al. Reduced macadamia nut quality is linked to wetter growing seasons but mitigated at higher elevations[J]. Journal of agriculture and food research, 2023, 12:1-10.
[7]	陶巧静, 臧丽丽, 刘蓉, 等. ¹³⁷Cs-γ辐射对葡萄种子发芽和幼苗生长及叶绿素荧光特性的影响[J]. 核农学报, 2015, 29(4):761-768. doi: 10.11869/j.issn.100-8551.2015.04.0761
[8]	胡瑶, 张跃龙, 李宏告, 等. ⁶⁰Co-γ辐射对矾根幼苗生理特性的影响[J]. 核农学报, 2020, 34(3):453-459. doi: 10.11869/j.issn.100-8551.2020.03.0453
[9]	UENO K, NAGAYOSHI S, IMAKIIRE S, et al. Breeding of new cultivar ‘Aladdin 2’ through stepwise improvements of cv. ‘Jimba’ using ion beam re-irradiation[J]. Horticultural research, 2013, 12(3):245-254.
[10]	宋恒, 王长泉, 巩向忠. γ射线辐照杜鹃试管苗诱发突变体的研究[J]. 核农学报, 2003(5):347-349.
[11]	董超, 尹静, 孔祥强. ⁶⁰Co-γ射线对不同陆地棉品种辐照效应和耐旱突变体筛选[J]. 核农学报, 2018, 32(9):1677-1683. doi: 10.11869/j.issn.100-8551.2018.09.1677
[12]	徐佳琦, 戚嘉敏, 朱雯, 等. ⁶⁰Co-γ辐射对洋紫荆种子发芽及幼苗生长的影响[J]. 林业与环境科学, 2016, 32(5):58-62.
[13]	KIM J H, CHUNG B Y, KIM J S, et al. Effects of in planta gamma-irradiation on growth, photosynthesis, and antioxidative capacity of red pepper (Capsicum annuum L.) plants[J]. Journal of plant biology, 2005, 48(1):47-56
[14]	李春牛, 李先民, 黄展文, 等. ⁶⁰Co-γ射线辐照对茉莉花种子萌发和幼苗生长及生理的影响[J]. 热带作物学报, 2022, 43(1):119-127. doi: 10.3969/j.issn.1000-2561.2022.01.016
[15]	叶力瑕, 牛耀星, 王燕, 等. 低剂量电子束辐照对徐香猕猴桃生理品质与氧化酶的影响[J]. 食品工业科技, 2023(6):358-365.
[16]	于明, 王丹, 王钢, 等. 电子束辐照对天麻粉灭菌效果及品质的影响[J]. 核农学报, 2022, 36(11):2175-2182. doi: 10.11869/j.issn.100-8551.2022.11.2175
[17]	易立飒, 李文锋, 崔之益, 等. ⁶⁰Co-γ辐照对广宁红花油茶种子发芽及幼苗生长的影响[J]. 华南农业大学学报, 2014, 35(5):93-97.
[18]	李秀芬, 张德顺, 吴福兰, 等. ⁶⁰Co-γ辐照对木槿种子发芽及幼苗生长的影响[J]. 核农学报, 2009, 23(3):450-453. doi: 10.11869/hnxb.2009.03.0450
[19]	陈令会, 洪莉, 董军, 等. ¹³⁷Cs-γ辐射对甜樱桃种子及接穗的影响效应[J]. 浙江农业科学, 2022, 63(7):1475-1477,1481. doi: 10.16178/j.issn.0528-9017.20220161
[20]	叶盈, 刘志高, 梁立军, 等. ¹³⁷Cs-γ射线辐射对威灵仙种子发芽及幼苗生长的影响[J]. 核农学报, 2020, 34(08):1621-1630.
[21]	倪书邦, 贺熙勇, 孔广红, 等. ⁶⁰Co辐照对澳洲坚果种子萌发与幼苗形态的影响[J]. 中国农学通报, 2011, 27(22):103-108.
[22]	谭瑗瑗, 汪庆, 富昊伟, 等. 利用花培与辐照诱变培育粳型两系不育系江79S[J]. 核农学报, 2022, 36(6):1073-1079. doi: 10.11869/j.issn.100-8551.2022.06.1073
[23]	WU J L, WU C, LEI C. Chemical-and irradiation-induced mutants of indica rice IR64 for forward and reverse genetics[J]. Plant molecular biology, 2005, 59(1):85-97.
[24]	桂仁意, 刘亚迪, 郭小勤, 等. 不同剂量¹³⁷Co-γ辐射对毛竹幼苗叶片叶绿素荧光参数的影响[J]. 植物学报, 2010, 45(1):66-72.
[25]	MELKI M, MAROUANI A. Effects of gamma rays irradiation on seed germination and growth of hard wheat[J]. Environmental chemistry letters, 2010, 8(4):307-310.
[26]	胡泊, 宾丽慧, 容敏坚, 等. 淮山药零余子⁶⁰Co-γ辐射的诱变效应[J]. 热带作物学报, 2023, 44(5):986-993. doi: 10.3969/j.issn.1000-2561.2023.05.014
[27]	LAANEN P, SAENEN E, MYSARA M, et al. Changes in DNA methylation in Arabidopsis thaliana plants exposed over multiple generations to gamma radiation[J]. Frontiers plant science, 2021, 12:611783.
[28]	林兵, 钟淮钦, 黄敏玲, 等. ⁶⁰Co-γ射线对荷兰鸢尾的辐照效应[J]. 核农学报, 2010, 24(1):50-54. doi: 10.11869/hnxb.2010.01.0050
[29]	KIM K N, SONG M A, HAN S H, et al. Inactivation of Xanthomonas citri subspcitri and effect on infection of Citrus canker by gamma irradiation[J]. Research in plant disease, 2014, 20(4):283-288.

参数	含义
叶片长度	真叶从叶柄端到叶尖的直线长度，不包含叶柄
叶片宽度	真叶最宽处的直线宽度
株高	幼苗从茎基部到顶部之间的垂直距离
茎粗	幼苗茎干地下部分和地上部分临界处的直径

参数	含义
叶片长度	真叶从叶柄端到叶尖的直线长度，不包含叶柄
叶片宽度	真叶最宽处的直线宽度
株高	幼苗从茎基部到顶部之间的垂直距离
茎粗	幼苗茎干地下部分和地上部分临界处的直径

辐射剂量/ Gy	叶缘刺			叶片长			叶片宽
辐射剂量/ Gy	3月	6月	9月	3月	6月	9月	3月	6月	9月
0	2.67±0.58a	3.0±0.00a	2.67±0.58a	11.03±0.47a	14.17±0.67a	16.9±0.30a	2.33±0.15a	2.63±0.15a	2.77±0.25a
50	2.67±0.58a	2.33±0.58ab	2.67±0.58a	11.17±1.23a	11.23±0.55c	14.7±0.95b	2.2±0.10ab	2.5±0.10ab	2.5±0.10bc
100	2.33±0.58a	2±0.00b	2.33±0.58a	9.5±1.18b	13.2±0.56b	15.63±0.15b	2.0±0.10b	2.3±0.10b	2.83±0.25a
150	0.33±0.58b	0.67±0.58c	0.33±0.58b	7.83±0.85c	11.53±0.65c	12.97±1.04c	1.67±0.12c	2.07±0.06c	2.37±0.06c
200	2.00±0.00a	2.33±0.58ab	2.33±0.58a	6.67±0.15c	9.87±0.31d	12.83±0.68c	1.63±0.15c	2.0±0.10c	2.03±0.06d
250	2.67±0.58a	2.67±0.58ab	2.33±0.58a	5.13±0.31d	6.83±0.35e	8.17±0.49d	1.33±0.12d	1.7±0.17d	1.7±0.10e

辐射剂量/ Gy	叶缘刺			叶片长			叶片宽
辐射剂量/ Gy	3月	6月	9月	3月	6月	9月	3月	6月	9月
0	2.67±0.58a	3.0±0.00a	2.67±0.58a	11.03±0.47a	14.17±0.67a	16.9±0.30a	2.33±0.15a	2.63±0.15a	2.77±0.25a
50	2.67±0.58a	2.33±0.58ab	2.67±0.58a	11.17±1.23a	11.23±0.55c	14.7±0.95b	2.2±0.10ab	2.5±0.10ab	2.5±0.10bc
100	2.33±0.58a	2±0.00b	2.33±0.58a	9.5±1.18b	13.2±0.56b	15.63±0.15b	2.0±0.10b	2.3±0.10b	2.83±0.25a
150	0.33±0.58b	0.67±0.58c	0.33±0.58b	7.83±0.85c	11.53±0.65c	12.97±1.04c	1.67±0.12c	2.07±0.06c	2.37±0.06c
200	2.00±0.00a	2.33±0.58ab	2.33±0.58a	6.67±0.15c	9.87±0.31d	12.83±0.68c	1.63±0.15c	2.0±0.10c	2.03±0.06d
250	2.67±0.58a	2.67±0.58ab	2.33±0.58a	5.13±0.31d	6.83±0.35e	8.17±0.49d	1.33±0.12d	1.7±0.17d	1.7±0.10e

辐射剂量/Gy	株高			茎粗
辐射剂量/Gy	3月	6月	9月	3月	6月	9月
0	16.03±0.59a	21.9±0.56a	25.9±0.36a	2.43±0.06a	2.8±0.20a	3.4±0.20a
50	15.17±0.35a	19.7±1.01b	25.43±1.06a	2.30±0.10a	2.40±0.10b	2.8±0.20b
100	10.47±1.10b	13.6±0.75c	16.63±0.96b	2.07±0.06b	2.37±0.06b	2.63±0.06b
150	10.90±0.66b	11.93±0.51d	14.57±0.87c	1.90±0.10b	2.17±0.06c	2.33±0.06c
200	9.93±0.51b	10.87±0.31d	13.2±0.36d	1.60±0.17c	1.83±0.06d	2.1±0.10c
250	7.47±0.65c	8.43±0.47e	9.4±0.46e	1.37±0.15d	1.57±0.06e	1.73±0.12d

Effect of ¹³⁷Cs-γ Irradiation on Seed Germination and Scion Growth in Macadamia Nut

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	辐射剂量
	0 Gy	10 Gy	25 Gy	50 Gy	100 Gy	200 Gy
成活率/%	50.67±4.16a	25.33±5.03b	15.33±3.05c	6.67±1.15d	1.33±1.15de	0±0e
长度/cm	30±4.04a	22.87±1.4b	17.6±0.85c	10.1±1.45d	0±0e	0±0e

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