SNP Molecular Markers: Research and Its Application in Crop Variety Identification

doi:10.11924/j.issn.1000-6850.casb2023-0683

Abstract

Abstract:

Crop variety identification is an important guarantee for breeding and promoting excellent varieties, and the appropriate detection method is the key for the accurate identification of varieties. With the development of molecular marker techniques, the third-generation molecular marker SNP has gradually been applied in the field of variety identification. This paper outlines the characteristics of SNP molecular markers, analyses the features and applicability of five commonly used high-throughput detection methods in crop research, including high resolution melting, competitive allele specific PCR, gene chips, sequencing and genotyping by target sequencing. The research and application of SNP markers in the identification of variety authenticity, purity testing, and analysis of genetic relationships and classification are summarized as well, in order to provide technical reference for subsequent variety identification.

Key words: SNP marks, high-throughput genotyping, authenticity identification, purity test, genetic relationship analysis

TIAN Haiyan, ZHANG Haina, WANG Yongqiang, ZHOU Yongping, ZHANG Yinglu. SNP Molecular Markers: Research and Its Application in Crop Variety Identification[J]. Chinese Agricultural Science Bulletin, 2024, 40(6): 115-121.

Figures/Tables 1

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