Growth-promoting Effect of Bacillus velenzensis GDND-2 on Tobacco

doi:10.11924/j.issn.1000-6850.casb2024-0567

Abstract

Abstract:

Bacillus velenzensis GDND-2 could effectively control tobacco Fusarium root rot. In order to elucidate its growth-promoting effects on tobacco, specific primers were used to amplify relevant growth-promoting genes. The capabilities for indole-3-acetic acid (IAA) production, siderophore secretion, and ACC deaminase activity were tested by Sackowski colorimetry, CAS agar plate assays, and sole nitrogen source methods respectively. The phosphorus and potassium solubilization capacities were detected by transparent circle method. Defense enzyme activities in tobacco after GDND-2 treatment were measured by assay kits. The growth-promoting effects of GDND-2 on tobacco were verified by potting experiment. Results revealed that GDND-2 possessed the growth-promoting gene ysnE and exhibited strong IAA production capacity, reaching 24.16 mg/L after fermentation for 36 h. The strain demonstrated solubilization of organic phosphorus, inorganic phosphorus, and potassium. GDND-2 fermentation broth significantly enhanced peroxidase (POD) activity in tobacco leaves, showing a 707.88% increase in POD activity after 96 h of treatment. The strain displayed remarkable growth-promoting effect on tobacco seedlings, increasing fresh weight by over 87.21% and root length by more than 75.61%. So Bacillus velezensis GDND-2 demonstrated excellent IAA secretion capacity and significantly promoted tobacco seedling growth, establishing itself as a high-quality biocontrol strain with superior characteristics.

Key words: biocontrol bacteria, Bacillus velenzensis GDND-2, growth-promoting genes, growth-promoting characteristics, IAA, tobacco, defense enzymes, growth-promoting effect

SHEN Huifang, YANG Qiyun, ZHANG Jingxin, PU Xiaoming, SUN Dayuan, LIU Pingping, LIN Birun. Growth-promoting Effect of Bacillus velenzensis GDND-2 on Tobacco[J]. Chinese Agricultural Science Bulletin, 2025, 41(16): 14-20.

Figures/Tables 10

References 32

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促生物质	目的基因	引物序列(5'→3')		文献
促生物质	目的基因	F	R	文献
吲哚乙酸	ysnE	GGCTGTGAACCTTTGCTATG	GCTGTTCGGGTCCTCTTTAT	[21]
铁载体	dhbC	ATGTTGGATCAAAACGTTAT	TCATATGTGATCCACGCCCA	[22]
ACC脱氨酶	acdS	ATCGGCGGCATCCAGWSNAAYCANAC	GGCACGCCGCCCARRTGNRCRTA	[23]

促生物质	目的基因	引物序列(5'→3')		文献
促生物质	目的基因	F	R	文献
吲哚乙酸	ysnE	GGCTGTGAACCTTTGCTATG	GCTGTTCGGGTCCTCTTTAT	[21]
铁载体	dhbC	ATGTTGGATCAAAACGTTAT	TCATATGTGATCCACGCCCA	[22]
ACC脱氨酶	acdS	ATCGGCGGCATCCAGWSNAAYCANAC	GGCACGCCGCCCARRTGNRCRTA	[23]

处理	发芽势/%	发芽率/%	根长/mm
无菌体上清液	95.00±1.26a	95.40±1.36a	16.64±0.35a
菌悬液	95.60±1.02a	96.00±1.22a	16.42±0.22a
发酵液	94.40±1.74a	95.20±1.30a	16.56±0.26a
NA(CK)	95.20±1.17a	95.80±1.10a	16.78±0.33a

处理	发芽势/%	发芽率/%	根长/mm
无菌体上清液	95.00±1.26a	95.40±1.36a	16.64±0.35a
菌悬液	95.60±1.02a	96.00±1.22a	16.42±0.22a
发酵液	94.40±1.74a	95.20±1.30a	16.56±0.26a
NA(CK)	95.20±1.17a	95.80±1.10a	16.78±0.33a

处理	根长/cm	株高/cm	最大叶长/cm	最大叶宽/cm	鲜重/mg	干重/mg
无菌体上清液	26.18±5.26a	9.01±0.98a	25.15±2.89a	18.68±2.56a	127.92±27.80a	6.16±2.08a
菌悬液	25.20±2.15a	9.23±0.69a	25.57±2.63a	19.38±1.88a	136.42±36.92a	6.25±2.20a
发酵液	26.54±3.68a	9.39±1.00a	26.21±2.03a	19.84±2.38a	144.33±33.66a	6.51±1.98a
NA(CK)	14.35±2.79b	7.71±0.61b	15.03±1.34b	13.58±1.70b	68.33±14.71b	3.33±0.75b