Comparison of Salt Tolerance at Seedling Stage and Analysis of Physiological Response Mechanism in Different Peanut Varieties

doi:10.11924/j.issn.1000-6850.casb2025-0713

Abstract

Abstract:

To compare the salt tolerance of 8 peanut varieties in Xinbaihua series under salt stress and analyze their physiological response mechanism. In this study, different peanut varieties were treated by hydroponics for 7 days respectively under 300 mmol/L NaCl stress. The plant height, biomass (fresh and dry weight) and root morphological indicators of each variety were measured, and the salt tolerance was comprehensively evaluated by membership function methods. Based on above results, 4 varieties with the greatest differences in salt tolerance were selected, and the physiological indexes, such as leaf chlorophyll content, reactive oxygen species (ROS) content and antioxidant enzyme activity were determined. The results showed that salt stress inhibited the plant height, biomass and root growth of each variety significantly. Among them, the inhibition of ‘Xinbaihua 25’ was the lowest, and its plant height, fresh weight, dry weight and total root length decreased by 14.29%, 53.69%, 29.64% and 43.63%, respectively; while ‘Xinbaihua 15’ was the most severely suppressed, corresponding indicators significantly decreased by 32.19%, 68.94%, 37.72% and 54.82%. The comprehensive evaluation results combined with membership function showed that the salt tolerance of ‘Xinbaihua 25’ was the strongest, followed by ‘Xinbaihua 27’, and the salt tolerance of ‘Xinbaihua 15’ and ‘Xinbaihua 17’ were the worst. The results of physiological indexes showed that compared with ‘Xinbaihua 15’ and ‘Xinbaihua 17’, the contents of O₂^-, H₂O₂ and MDA in leaves of ‘Xinbaihua 25’ and ‘Xinbaihua 27’ with strong salt tolerance were significantly decreased, while the contents of chlorophyll and the activities of POD, CAT and APX were increased. This phenomenon showed that peanut varieties with strong salt tolerance had stronger antioxidant capacity and photosynthetic capacity, thus alleviating the inhibition of salt stress on peanut growth. In short, the salt tolerance of 8 peanut varieties are compared and analyzed systematically, which provide theoretical basis and germplasm resources for further studying the molecular mechanism of their response to salt stress and cultivating salt-tolerant varieties.

Key words: peanut, salt stress, salt tolerance, reactive oxygen species, antioxidant enzyme

CLC Number:

S565.2

SHAO Mengyao, KONG Xiangjun, JIA Peipei, LI Hengbin, YAN Sihui, SUN Chenxin, LI Lijie, LI Zengqiang, ZHANG Zhiyong. Comparison of Salt Tolerance at Seedling Stage and Analysis of Physiological Response Mechanism in Different Peanut Varieties[J]. Chinese Agricultural Science Bulletin, 2026, 42(14): 46-56.

Figures/Tables 11

References 36

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品种（品系）	处理	总根长/cm	根表面积/cm²	根体积/cm³
新百花13	CK	462.58±78.97cd	118.43±27.49cd	2.43±0.39cde
新百花13	NaCl	243.13±72.93ghi	49.36±13.63f	1.15±0.56ef
新百花15	CK	477.68±99.04c	112.10±49.12cd	3.25±1.24bc
新百花15	NaCl	215.81±24.62hi	53.59±14.41f	0.92±0.21f
新百花16	CK	448.13±88.66cde	141.85±12.14bc	4.26±0.67ab
新百花16	NaCl	239.09±13.95ghi	59.88±3.98f	1.17±0.16ef
新百花17	CK	787.78±67.86a	224.13±24.96a	5.08±1.61a
新百花17	NaCl	223.87±44.11hi	56.67±5.32f	1.15±0.09ef
新百花21	CK	693.74±68.39ab	212.46±13.57a	4.60±1.09a
新百花21	NaCl	366.80±12.16def	46.79±6.90f	1.23±0.32ef
新百花23	CK	599.35±72.90b	153.08±31.43b	2.73±0.91cd
新百花23	NaCl	181.46±17.90hi	100.37±15.42de	1.88±0.36def
新百花25	CK	275.15±5.68fgh	64.65±2.68f	1.38±0.39ef
新百花25	NaCl	155.10±83.49i	49.83±10.64f	1.21±0.12ef
新百花27	CK	650.51±70.76b	170.57±8.73b	3.23±0.73bc
新百花27	NaCl	346.63±40.67efg	70.62±3.59ef	1.36±0.08ef

品种（品系）	处理	总根长/cm	根表面积/cm²	根体积/cm³
新百花13	CK	462.58±78.97cd	118.43±27.49cd	2.43±0.39cde
新百花13	NaCl	243.13±72.93ghi	49.36±13.63f	1.15±0.56ef
新百花15	CK	477.68±99.04c	112.10±49.12cd	3.25±1.24bc
新百花15	NaCl	215.81±24.62hi	53.59±14.41f	0.92±0.21f
新百花16	CK	448.13±88.66cde	141.85±12.14bc	4.26±0.67ab
新百花16	NaCl	239.09±13.95ghi	59.88±3.98f	1.17±0.16ef
新百花17	CK	787.78±67.86a	224.13±24.96a	5.08±1.61a
新百花17	NaCl	223.87±44.11hi	56.67±5.32f	1.15±0.09ef
新百花21	CK	693.74±68.39ab	212.46±13.57a	4.60±1.09a
新百花21	NaCl	366.80±12.16def	46.79±6.90f	1.23±0.32ef
新百花23	CK	599.35±72.90b	153.08±31.43b	2.73±0.91cd
新百花23	NaCl	181.46±17.90hi	100.37±15.42de	1.88±0.36def
新百花25	CK	275.15±5.68fgh	64.65±2.68f	1.38±0.39ef
新百花25	NaCl	155.10±83.49i	49.83±10.64f	1.21±0.12ef
新百花27	CK	650.51±70.76b	170.57±8.73b	3.23±0.73bc
新百花27	NaCl	346.63±40.67efg	70.62±3.59ef	1.36±0.08ef

	株高	鲜重	干重	总根长	根表面积
鲜重	0.785^**
干重	0.713^**	0.892^**
总根长	0.663^**	0.716^**	0.627^**
根表面积	0.630^**	0.695^**	0.569^*	0.923^**
根体积	0.679^**	0.669^**	0.457	0.876^**	0.948^**

	株高	鲜重	干重	总根长	根表面积
鲜重	0.785^**
干重	0.713^**	0.892^**
总根长	0.663^**	0.716^**	0.627^**
根表面积	0.630^**	0.695^**	0.569^*	0.923^**
根体积	0.679^**	0.669^**	0.457	0.876^**	0.948^**

品种（品系）		隶属函数值(μ)							耐盐性综合评价值(D)
品种（品系）		μ₁		μ₂		μ₃	μ₄		值		排名
新百花13	0.40		0.26		0.61			0.74		0.42		6
新百花15	0.00		0.20		0.55			0.32		0.16		8
新百花16	0.47		0.47		0.70			0.35		0.49		5
新百花17	0.40		0.03		0.74			0.33		0.34		7
新百花21	0.46		0.82		0.84			1.00		0.66		3
新百花23	0.84		0.35		0.00			0.56		0.56		4
新百花25	0.64		1.00		0.69			0.00		0.69		1
新百花27	1.00		0.00		1.00			0.45		0.67		2