Construction of a High-density Genetic Map and QTL Mapping for Sex and Growth Traits in Bellamya purificata

doi:10.11924/j.issn.1000-6850.casb2026-0199

Abstract

Abstract:

In order to carry out molecular marker-assisted breeding and fine mapping of related functional genes of Bellamya purificata, 42 individuals of the recombinant inbred line ‘RIL3’ of B. purificata were used as the mapping population. Based on the whole genome resequencing technology, the whole genome SNP markers were mined and screened. The high-quality SNP markers obtained by mining and screening were used to construct a high-density genetic map using HighMap software. R/qtl software was used to locate QTLs related to gender and growth traits, and candidate genes were screened by genome annotation and functional enrichment analysis. A high-density genetic map containing of 1503 Bin markers with a total map distance of 1115.71 cM was successfully constructed, with an average marker spacing of 0.81 cM. Four QTLs for sex (XB), weight (TZ) and shell width (KK) were mapped on LG2, and the sex QTL explained 17.32 % of the phenotypic variation. Candidate gene analysis showed that the gender QTL interval was enriched in genes related to transmembrane signal transduction, protein dephosphorylation and lipid metabolism, and the growth trait QTL interval was involved in chitin metabolism, kinase signaling and basal metabolic pathways. The results indicate that the sex determination of B. purificata may be regulated by a non-classical signal network with receptor tyrosine phosphatase as the core, and the growth traits are affected by multiple genes, which provide a key genetic basis for the molecular mechanism analysis and molecular breeding of sex and growth traits of this species.

Key words: Bellamya purificata, genetic map, QTL, sex determination, growth traits

CLC Number:

S966.28

WU Hao, MA Xueyan, WEN Haibo, YANG Kaixuan, YUAN Kai, MA Benhe, PAN Xianhui, JIN Wu. Construction of a High-density Genetic Map and QTL Mapping for Sex and Growth Traits in Bellamya purificata[J]. Chinese Agricultural Science Bulletin, 2026, 42(17): 189-195.

Figures/Tables 7

References 36

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Sample ID	Raw reads	clean reads	clean bases/Gb	Q20/%	Q30/%	Clean bases ratio/%	GC/%
Female parent ♀	259.99	259.89	38.95	98.02	93.76	99.87	34.62
Male parent ♂	204.22	204.13	30.6	98.01	93.6	99.9	34.21
Offspring average	74.34	74.31	11.14	98.04	93.73	99.87	34.63

Sample ID	Raw reads	clean reads	clean bases/Gb	Q20/%	Q30/%	Clean bases ratio/%	GC/%
Female parent ♀	259.99	259.89	38.95	98.02	93.76	99.87	34.62
Male parent ♂	204.22	204.13	30.6	98.01	93.6	99.9	34.21
Offspring average	74.34	74.31	11.14	98.04	93.73	99.87	34.63

LG ID	Total Bin Marker	Total Distance(cM)	Average Distance(cM)	Max Gap(cM)	Gaps<5cM(%)
LG1	250	136.45	0.55	6.71	99.6
LG2	227	169.5	0.75	6.46	98.23
LG3	150	153.79	1.03	11.13	92.62
LG4	121	117.07	0.97	13.02	97.5
LG5	233	100.43	0.43	4.06	100
LG6	196	179.49	0.92	5.26	98.97
LG7	90	109.31	1.21	6.95	93.26
LG8	236	149.67	0.63	9.89	98.3
Total	1503	1115.71	0.81	13.02	97.31

LG ID	Total Bin Marker	Total Distance(cM)	Average Distance(cM)	Max Gap(cM)	Gaps<5cM(%)
LG1	250	136.45	0.55	6.71	99.6
LG2	227	169.5	0.75	6.46	98.23
LG3	150	153.79	1.03	11.13	92.62
LG4	121	117.07	0.97	13.02	97.5
LG5	233	100.43	0.43	4.06	100
LG6	196	179.49	0.92	5.26	98.97
LG7	90	109.31	1.21	6.95	93.26
LG8	236	149.67	0.63	9.89	98.3
Total	1503	1115.71	0.81	13.02	97.31

性状	QTL	连锁群	LOD	置信区间(cM)	加性效应	显性效应	解释变异
体重	TZ.1	LG2	2.943	10.721~11.911	0.560	-0.051	5.653
体重	TZ.2	LG2	2.680	8.339~8.339	0.530	-0.112	5.009
壳宽	KK	LG2	2.549	10.721~10.721	1.058	0.048	5.315
性别	XB	LG2	3.530	97.070~98.261	0.089	-0.564	17.320