Abstract:

Abstract: Hex（haematopoietically expressed homeobox, hex）gene has important function in vertebrate development.To study the function of hex gene in mammary gland development, the total RNA was extracted, hex gene was amplificated by RT-PCR and cloned into pMD18-T in the study.The result indicated that the open reading frame of hex gene was 816bp in length which could encode 271 amino acids,was 30KD molecular weight.There is complete similarity with the sequence published from GenBank.The homology of amino acids were 69.8%-97.0% with chicken,human,rat,xenopus and zebrafish.The phylogenetic tree of HEX protein from different species proved that there are seven differences in amino acid sequence between mouse and rat. HEX protein was conserved in sequence, it has important function in evolution.