Abstract:

Abstract: two kinds of artificial antigen for progesterone(P4) were synthesized and identified in this research. Progesterone-11alpha-hemisuccinate was conjugated with carrier protein Albumin Bovine V(BSA) and Albumin egg(OVA) by dicyclohexylcarbodiimide(DCC).Then,10 Balb/c mice were injected with immunogen P4-BSA to produce antibody against P4. The prepared artificial antigens were accredited by Ultra violet scan and the titer of antibody against P4 was detected by Elisa. The results show that the conjugation molecular ratios of P4 with BSA and OVA are 18:1 and 10:1 and the titer is as high as 1:25600.