Abstract:

Protoplast preparation and regeneration is one of the most important methods for constructing genetic transformation in Fusarium oxysporum f.sp. conglutinans. The culture time of mycelium, the enzymolysis time, the osmotic stabilizer typer and the osmotic stabilizer concentration were optimized, at last the author gained an efficient method for protoplast preparation and regeneration of F. oxysporum. Furthermore, the protoplast can regenerate on regeneration medium, the regeneration rate of the protoplast was 21.13%. The system provided an basic for F. oxysporum genetic transformation and pathogenicity analysis in molecular levels.