Abstract:

In order to explore In vitro gerbera leaves culture about adventitious shoot regeneration, improve adventitious shoot regeneration system of gerbera leaves in vitro. Adventitious shoot regeneration from detached leaves: the effects of factors on adventitious shoot formation on leaf explants of gerbera were examined, including the detaching pattern of leaf explants, basal medium and plant growth regulation. The results indicated that adventitious shoot formation occurred at the base of leaf petioles (detached and cut off from stock plant), leaves with petioles shortened to one-half in length did not form adventitious shoots, only form callus. Detached leaves which grown on half-strength MS containing 5.0 mg/L KT and 0.1 mg/L NAA medium formed adventitious shoot as follows: organ type regeneration rate was up to 60%~70%, organ type regeneration ratio was 1-2, organ occurring regeneration rate was 20%-30%, organ occurring regeneration ratio was 1-2. Organ type adventitious shoot formation from detached leaves mainly occurred during 4~12 days after inoculation of leaves detached from stock plant, and adventitious organ occurring adventitious shoot formation from detached leaves mainly occurred during 12-24 days after inoculation of leaves detached from stock plant. The culture medium supplemented with BA was more effective than KT. If the different concentrations for BA and KT, organ type regeneration rate were up to 60%-70%, organ type regeneration ratio was 4-5, organ occurring regeneration rate was 50%-60%, organ occurring regeneration ratio was 2-3.