Chinese Agricultural Science Bulletin ›› 2005, Vol. 21 ›› Issue (3): 84-84.
Special Issue: 玉米; 烟草种植与生产
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Lu Ruiju, Wang Yifei, Sun Yuefang, Zhou Runmei, Huang Jianhua
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Abstract: The vigor and rooting of plantlets derived from microspore culture of maize could be obviously improved by adding 1.0mg/L IBA, 0.05mg/L NAA, 1mg/l MET to rooting medium. The transplanting survival rate of plantlets could be increased to 90% through the procedure of hardening plantlets for 24~48h, growing of 5~7d with medium of perlit or vermiculite and then transplanting them to soil supplemented with 1/3 chicken excrement thoroughly decomposed. The survived plants with deep grown color-leaves and vigor roots could grow strongly in the field. The method for measurement of the length of stoma guard cell was proved to be a simple way of estimating ploidy of the regenerated plants.
Lu Ruiju, Wang Yifei, Sun Yuefang, Zhou Runmei, Huang Jianhua. Ploidy-estimating and Transplanting of regenerated-plants derived from microspore culture of maize[J]. Chinese Agricultural Science Bulletin, 2005, 21(3): 84-84.
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https://www.casb.org.cn/EN/Y2005/V21/I3/84