Abstract:

To find out the effects of ovine sperm acrosome reaction induced by zone pellucid, three experiments were designed: first, co-incubation non-capacited sperm with zone pellucid; second, co-incubation capacitation sperm with zone pellucid; third, adding with calcium ionophore A23187 after co-incubation non-capacited sperm with zone pellucid. The results showed that the rates of acrosome reaction induced by zone pellucid are not significant difference whether the spermatozoa were capacited, IA23187 don’t cooperate with zone pellucide to improve the rate of acrosome reaction.