Abstract:

Protease (including acid protease， alkaline protease and neutral protease) is the one of the most important enzymes in the feed compound enzymes. In this paper， acid protease， alkaline protease and neutral protease of the CHE feed were extracted and the assay of their activity were carried out. The results showed that the enzyme activity was not the same in all of them， acidic protease activity was the highest， the smallest one was alkaline protease， and the optimum temperature of acidic protein activity was about 40 ℃ and the maximum of acidic protein activity was 5.203ug / min， the optimum temperature of neutral protease was about 30 ℃ and the maximum of neutral protease was 2.9754 ug / min， while the optimum temperature of alkaline protease was about 25 ℃ and the maximum of alkaline protease was 0.4482 ug / min.